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Isolation and Partial Characterization of a Mutant of Escherichia coli Deficient in DNA Polymerase II

Campbell, Judith L. and Soll, Larry and Richardson, Charles C. (1972) Isolation and Partial Characterization of a Mutant of Escherichia coli Deficient in DNA Polymerase II. Proceedings of the National Academy of Sciences of the United States of America, 69 (8). pp. 2090-2094. ISSN 0027-8424. PMCID PMC426875. https://resolver.caltech.edu/CaltechAUTHORS:20190510-105529866

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Abstract

A mutant of Escherichia coli deficient in DNA polymerase II has been isolated from E. coli polA1 by mutagenesis with N-methyl-N′-nitro-N-nitrosoguanidine and assay of polymerase activity in extracts of survivors. The polA1 mutation was suppressed during mutagenesis by introduction of the suppressor, su7^+, into the parental strain. The mutant, HMS83 polA1 polB1, contains less than 0.5% of the normal levels of DNA polymerase II. The only polymerase activity detected in the mutant is DNA polymerase III. E. coli HMS83 grows normally at both 25 and 42°, and supports the growth of bacteriophages T4, T7, lambda, ϕX174, 186, P2, and fd. The polB mutation does not affect sensitivity to ultraviolet irradiation or recombination frequencies.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1073/pnas.69.8.2090DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC426875/PubMed CentralArticle
Additional Information:© 1972 National Academy of Sciences. Communicated by Eugene P. Kennedy, May 26, 1972. We thank Henry Mulcahy and Robert DiNello for their excellent assistance, and Dr. Hiroaki Shizuya for his helpful advice. This investigation was supported by research grants from the National Institutes of Health, United States Public Health Service Grant AI-06045, and Grant P486 from the American Cancer Society, Inc. C. C. R. is the recipient of a Public Health Service Research Career Program Award, no. GM-13,534.
Funders:
Funding AgencyGrant Number
NIHAI-06045
American Cancer SocietyP486
NIHGM-13534
Issue or Number:8
PubMed Central ID:PMC426875
Record Number:CaltechAUTHORS:20190510-105529866
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190510-105529866
Official Citation:Isolation and Partial Characterization of a Mutant of Escherichia coli Deficient in DNA Polymerase II Judith L. Campbell, Larry Soll, Charles C. Richardson Proceedings of the National Academy of Sciences Aug 1972, 69 (8) 2090-2094; DOI: 10.1073/pnas.69.8.2090
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95396
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:10 May 2019 22:05
Last Modified:03 Oct 2019 21:13

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