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Inhibition of Gene Expression by Homologous Double-Stranded RNA in a Drosophila melanogaster Cell Culture

Aravin, A. A. and Vagin, V. V. and Rozovsky, Ya. M. and Gvozdev, V. A. (2001) Inhibition of Gene Expression by Homologous Double-Stranded RNA in a Drosophila melanogaster Cell Culture. Russian Journal of Genetics, 37 (6). pp. 639-642. ISSN 1022-7954. https://resolver.caltech.edu/CaltechAUTHORS:20190513-140252620

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Abstract

Specific inhibition of gene expression by exogenous homologous double-stranded RNA (dsRNA) in invertebrates and in the early development of vertebrates is termed RNA interference. Cultured cells were cotransfected with reporter plasmids and dsRNA. The inhibitory effect on reporter gene expression depended on the extent of homology between dsRNA and the target gene. RNA interference was also studied in cells cotransfected with plasmids directing synthesis of sense and antisense RNAs. Production of antisense RNA only slightly inhibited expression of the reporter gene. Simultaneous expression of both sense and antisense RNAs caused by cotransfection by corresponding plasmids did not inhibit expression of the reporter construct.


Item Type:Article
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https://doi.org/10.1023/a:1016621207467DOIArticle
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Additional Information:© 2001 MAIK “Nauka/Interperiodica”. Russian Journal of Genetics, Vol. 37, No. 6, 2001, pp. 639–642. Translated from Genetika, Vol. 37, No. 6, 2001, pp. 779–783. Original Russian Text Copyright © 2001 by Aravin, Vagin, Rozovsky, Gvozdev. Received January 3, 2001. This work was supported by the Russian Foundation for Basic Research (project nos. 99-04-48561, 00-15-97896) and the Russian programs “Frontiers in Genetics” (project no. 99-1069) and “Russian Universities: Basic Research” (project no. 5244).
Funders:
Funding AgencyGrant Number
Russian Foundation for Basic Research99-04-48561
Russian Foundation for Basic Research00-15-97896
Russian Foundation for Basic Research99-1069
Russian Foundation for Basic Research5244
Subject Keywords:Gene Expression; Target Gene; Reporter Gene; Early Development; Specific Inhibition
Issue or Number:6
Record Number:CaltechAUTHORS:20190513-140252620
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190513-140252620
Official Citation:Aravin, A.A., Vagin, V.V., Rozovsky, Y.M. et al. Russian Journal of Genetics (2001) 37: 639. https://doi.org/10.1023/A:1016621207467
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95443
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 May 2019 21:16
Last Modified:03 Oct 2019 21:13

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