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The mitochondrial ND8 gene from Crithidia oncopelti is not pan-edited

Aravin, Alexey A. and Yurchenko, Vyacheslav Yu. and Merzlyak, Ekaterina M. and Kolesnikov, Alexander A. (1998) The mitochondrial ND8 gene from Crithidia oncopelti is not pan-edited. FEBS Letters, 431 (3). pp. 457-460. ISSN 0014-5793. https://resolver.caltech.edu/CaltechAUTHORS:20190513-143549674

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Abstract

RNA editing in trypanosomatid mitochondria is a process involving the insertion and deletion of uridine residues within the coding region of maxicircle messenger RNA transcripts. Twelve of the 17 known genes need editing to produce functional molecules. We have analyzed the predicted editing sites for the Crithidia oncopelti mitochondrial NADH‐ubiquinone oxidoreductase subunit 8 (ND8) gene based on known mRNAs from other trypanosomatid species. All studied ND8 mRNAs undergo editing throughout the coding (and 3′ non‐coding) sequences (pan‐editing). The 5′ part of the C. oncopelti ND8 gene undergoes editing (like in Leishmania tarentolae and Trypanosoma brucei) while the 3′ part of the pre‐edited gene corresponds to the 3′ part of edited ND8 mRNAs from other organisms. The organization of the ND8 gene in C. oncopelti mitochondrial DNA differs from all organisms investigated so far – this gene is not pan‐edited. We have also localized the guide RNA for cytochrome b between 9S rRNA and the ND8 gene. This RNA shows high homology to the gCYb‐II gene of L. tarentolae and the gCyb gene of Crithidia fasciculata. A hypothetical editing pattern for the cytochrome b gene in C. oncopelti maxicircles is proposed.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/s0014-5793(98)00813-8DOIArticle
Additional Information:© 1998 Federation of European Biochemical Societies. Received 17 June 1998. We would like to thank Dr. D.A. Maslov for his interest in our work and helpful discussion; Drs. N.S. Entelis, M.N. Merzlyak and I.A. Krascheninnikov for their critical reading of the manuscript and T.A. Yurchenko for her help with pictures. This work was supported by a grant from the Russian Foundation for Basic Research.
Funders:
Funding AgencyGrant Number
Russian Foundation for Basic ResearchUNSPECIFIED
Subject Keywords:Crithidia oncopelti; RNA editing; Maxicircle; ND8
Issue or Number:3
Record Number:CaltechAUTHORS:20190513-143549674
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190513-143549674
Official Citation:Aravin, Alexey A, Yurchenko, Vyacheslav Yu, Merzlyak, Ekaterina M and Kolesnikov, Alexander A( 1998), The mitochondrial ND8 gene from Crithidia oncopelti is not pan-edited, FEBS Letters, 431, doi: 10.1016/S0014-5793(98)00813-8
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95446
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 May 2019 21:41
Last Modified:03 Oct 2019 21:13

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