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Opposing Wnt Pathways Orient Cell Polarity during Organogenesis

Green, Jennifer L. and Inoue, Takao and Sternberg, Paul W. (2008) Opposing Wnt Pathways Orient Cell Polarity during Organogenesis. Cell, 134 (4). pp. 646-656. ISSN 0092-8674. PMCID PMC2603076. https://resolver.caltech.edu/CaltechAUTHORS:20190514-082856223

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Abstract

The orientation of asymmetric cell division contributes to the organization of cells within a tissue or organ. For example, mirror-image symmetry of the C. elegans vulva is achieved by the opposite division orientation of the vulval precursor cells (VPCs) flanking the axis of symmetry. We characterized the molecular mechanisms contributing to this division pattern. Wnts MOM-2 and LIN-44 are expressed at the axis of symmetry and orient the VPCs toward the center. These Wnts act via Fz/LIN-17 and Ryk/LIN-18, which control β-catenin localization and activate gene transcription. In addition, VPCs on both sides of the axis of symmetry possess a uniform underlying “ground” polarity, established by the instructive activity of Wnt/EGL-20. EGL-20 establishes ground polarity via a novel type of signaling involving the Ror receptor tyrosine kinase CAM-1 and the planar cell polarity component Van Gogh/VANG-1. Thus, tissue polarity is determined by the integration of multiple Wnt pathways.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.cell.2008.06.026DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2603076PubMed CentralArticle
ORCID:
AuthorORCID
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2008 Elsevier Inc. Received 30 October 2007, Revised 23 April 2008, Accepted 10 June 2008, Available online 21 August 2008. We thank Gladys Medina, Barbara Perry, and Shahla Gharib for technical assistance, members of our lab for helpful discussions, Andrea Choe for artistic input, and Marianne Bronner-Fraser, Scott Fraser, Cheryl Van Buskirk, Mihoko Kato, Elissa Hallem, and Adeline Seah for critically reading the manuscript. Many strains used in this study were provided by the Caenorhabditis Genetics Center, funded by the National Institutes of Health, National Center for Research Resources. We are grateful to the late Peter Snow for his valuable assistance in making our CAM-1 antibody. P.W.S. is an investigator with the Howard Hughes Medical Institute, who supported this work, and J.L.G. was supported by the Moore Foundation Fellowship for graduate study toward the Doctor of Philosophy degree in Biology at the California Institute of Technology.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Subject Keywords:CELLBIO; DEVBIO; SIGNALING
Issue or Number:4
PubMed Central ID:PMC2603076
Record Number:CaltechAUTHORS:20190514-082856223
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190514-082856223
Official Citation:Jennifer L. Green, Takao Inoue, Paul W. Sternberg, Opposing Wnt Pathways Orient Cell Polarity during Organogenesis, Cell, Volume 134, Issue 4, 2008, Pages 646-656, ISSN 0092-8674, https://doi.org/10.1016/j.cell.2008.06.026. (http://www.sciencedirect.com/science/article/pii/S0092867408007782)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95468
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 May 2019 21:16
Last Modified:03 Oct 2019 21:13

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