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Genetic Analysis of Dauer Formation in Caenorhabditis briggsae

Inoue, Takao and Ailion, Michael and Poon, Shirley and Kim, Hannah K. and Thomas, James H. and Sternberg, Paul W. (2007) Genetic Analysis of Dauer Formation in Caenorhabditis briggsae. Genetics, 177 (2). pp. 809-818. ISSN 0016-6731. PMCID PMC2034645. https://resolver.caltech.edu/CaltechAUTHORS:20190514-084400196

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Abstract

Molecular changes that underlie evolutionary changes in behavior and physiology are not well understood. Dauer formation in Caenorhabditis elegans is a temperature-sensitive process controlled through a network of signaling pathways associated with sensory neurons and is potentially an excellent system in which to investigate molecular changes in neuronal function during evolution. To begin to investigate the evolution of dauer formation in the genus Caenorhabditis at the molecular level, we isolated dauer-formation mutations in C. briggsae, a species closely related to the model organism C. elegans. We identified mutations in orthologs of C. elegans genes daf-2 (insulin receptor), daf-3 (Smad), and daf-4 (TGF-β type 2 receptor), as well as genes required for formation of sensory cilia. Phenotypic analyses revealed that functions of these genes are conserved between C. elegans and C. briggsae. Analysis of C. briggsae mutations also revealed a significant difference between the two species in their responses to high temperatures (>26°). C. elegans is strongly induced to form dauers at temperatures above 26°, near the upper limit for growth of C. elegans. In contrast, C. briggsae, which is capable of growth at higher temperatures than C. elegans, lacks this response.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1534/genetics.107.078857DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034645PubMed CentralArticle
ORCID:
AuthorORCID
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2007 by the Genetics Society of America. Manuscript received July 13, 2007; Accepted for publication July 18, 2007. We thank R. Johnsen and D. Baillie for C. briggsae mutants and mapping data. We thank S. Gharib and B. P. Gupta for technical assistance and useful reagents. We thank E. Hallem, X. Wang, and J. Srinivasan for comments on the manuscript. T.I. was supported by fellowship DRG-1646 from the Damon Runyon Cancer Research Foundation. This work was supported by HHMI, where P.W.S. is an investigator. Some nematode strains used in this work were provided by the Caenorhabditis Genetics Center, which is funded by the National Institutes of Health National Center for Research Resources (NCRR).
Funders:
Funding AgencyGrant Number
Damon Runyon Cancer Research FoundationDRG-1646
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NIHUNSPECIFIED
Issue or Number:2
PubMed Central ID:PMC2034645
Record Number:CaltechAUTHORS:20190514-084400196
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190514-084400196
Official Citation:Genetic Analysis of Dauer Formation in Caenorhabditis briggsae. Takao Inoue, Michael Ailion, Shirley Poon, Hannah K. Kim, James H. Thomas and Paul W. Sternberg. GENETICS October 1, 2007 vol. 177 no. 2 809-818; https://doi.org/10.1534/genetics.107.078857
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95470
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 May 2019 21:13
Last Modified:03 Oct 2019 21:13

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