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The L-Type Cyclin CYL-1 and the Heat-Shock-Factor HSF-1 Are Required for Heat-Shock-Induced Protein Expression in Caenorhabditis elegans

Hajdu-Cronin, Yvonne M. and Chen, Wen J. and Sternberg, Paul W. (2004) The L-Type Cyclin CYL-1 and the Heat-Shock-Factor HSF-1 Are Required for Heat-Shock-Induced Protein Expression in Caenorhabditis elegans. Genetics, 168 (4). pp. 1937-1949. ISSN 0016-6731. PMCID PMC1448743. doi:10.1534/genetics.104.028423. https://resolver.caltech.edu/CaltechAUTHORS:20190514-092613986

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Abstract

In a screen for suppressors of activated GOA-1 (Gα_o) under the control of the hsp-16.2heat-shock promoter, we identified three genetic loci that affected heat-shock-induced GOA-1 expression. The cyl-1 mutants are essentially wild type in appearance, while hsf-1and sup-45 mutants have egg-laying defects. The hsf-1 mutation also causes a temperature-sensitive developmental arrest, and hsf-1 mutants have decreased life span. Western analysis indicated that mutations in all three loci suppressed the activated GOA-1 transgene by decreasing its expression. Heat-shock-induced expression of hsp-16.2 mRNA was reduced in cyl-1 mutants and virtually eliminated in hsf-1 and sup-45 mutants, as compared to wild-type expression. The mutations could also suppress other transgenes under heat-shock control. cyl-1 and sup-45, but not hsf-1, mutations suppressed a defect caused by a transgene not under heat-shock control, suggesting a role in general transcription or a post-transcriptional aspect of gene expression. hsf-1 encodes the C. elegans homolog of the human heat-shock factor HSF1, and cyl-1 encodes a cyclin most similar to cyclin L. We believe HSF-1 acts in heat-shock-inducible transcription and CYL-1 acts more generally in gene expression.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1534/genetics.104.028423DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1448743PubMed CentralArticle
ORCID:
AuthorORCID
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2004 by the Genetics Society of America. Manuscript received March 10, 2004; Accepted for publication July 29, 2004. We thank C. Bastiani for C. elegans GOA-1 antibody, M. C. Hresko and R. Waterston for MH16 anti-paramyosin antibody, the C. elegans Genetics Center for strains, A. Coulson for cosmids used in positional cloning, and Y. Kohara for cDNA clones. We thank H. Yu, G. Schindelman, E. Schwarz, N. Moghal, L. R. Garcia, C. van Buskirk, C. Bastiani, R. Lee, B. Gupta, and T. Inoue for advice and helpful discussions. We thank E.-S. David, R. Pant, and C. Dionne for guidance and E. Rothenberg for use of her facility for Q-PCR. C. Cronin provided the software for analyzing the length of worms. Most mapping information was obtained from WormBase (http://www.wormbase.org). Research was supported by the Howard Hughes Medical Institute, with which P.W.S. is an investigator. W.J.C. was an Amgen graduate Fellow. Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under accession nos. AY557405, AY559747, and AY559748.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
AmgenUNSPECIFIED
Issue or Number:4
PubMed Central ID:PMC1448743
DOI:10.1534/genetics.104.028423
Record Number:CaltechAUTHORS:20190514-092613986
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190514-092613986
Official Citation:The L-Type Cyclin CYL-1 and the Heat-Shock-Factor HSF-1 Are Required for Heat-Shock-Induced Protein Expression in Caenorhabditis elegans. Yvonne M. Hajdu-Cronin, Wen J. Chen and Paul W. Sternberg. GENETICS December 1, 2004 vol. 168 no. 4 1937-1949; https://doi.org/10.1534/genetics.104.028423
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95475
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 May 2019 21:01
Last Modified:16 Nov 2021 17:12

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