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The small ubiquitin-like modifier (SUMO) is required for gonadal and uterine-vulval morphogenesis in Caenorhabditis elegans

Broday, Limor and Kolotuev, Irina and Didier, Christine and Bhoumik, Anindita and Gupta, Bhagwati P. and Sternberg, Paul W. and Podbilewicz, Benjamin and Ronai, Ze’ev (2004) The small ubiquitin-like modifier (SUMO) is required for gonadal and uterine-vulval morphogenesis in Caenorhabditis elegans. Genes and Development, 18 (19). pp. 2380-2391. ISSN 0890-9369. PMCID PMC522988. https://resolver.caltech.edu/CaltechAUTHORS:20190514-100517710

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Abstract

The small ubiquitin-like modifier (SUMO) modification alters the subcellular distribution and function of its substrates. Here we show the major role of SUMO during the development of the Caenorhabditis elegans reproductive system. smo-1 deletion mutants develop into sterile adults with abnormal somatic gonad, germ line, and vulva. SMO-1::GFP reporter is highly expressed in the somatic reproductive system. smo-1 animals lack a vulval-uterine connection as a result of impaired ventral uterine π-cell differentiation and anchor cell fusion. Mutations in the LIN-11 LIM domain transcription factor lead to a uterine phenotype that resembles the smo-1 phenotype. LIN-11 is sumoylated, and its sumoylation is required for its activity during uterine morphogenesis. Expression of a SUMO-modified LIN-11 in the smo-1 background partially rescued π-cell differentiation and retained LIN-11 in nuclear bodies. Thus, our results identify the reproductive system as the major SUMO target during postembryonic development and highlight LIN-11 as a physiological substrate whose sumoylation is associated with the formation of a functional vulval-uterine connection.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/gad.1227104DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC522988PubMed CentralArticle
http://genesdev.cshlp.org/content/18/19/2380/suppl/DC1PublisherSupporting Information
ORCID:
AuthorORCID
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2004 by Cold Spring Harbor Laboratory Press. Received May 28, 2004; revised version accepted August 9, 2004. We thank the C. elegans Gene Knockout Project team at OMRF for generating the smo-1 null strain. We thank the Caernorhabditis Genetics Center (CGC) for providing strains, Reuven Agami for the kind gift of the pSUPER construct, Ke Shuai for the PIAS3 plasmid, Susan Strome for the SP56 antibody, and Chelly Hresko and Bob Waterston for the MH27 antibody. We also thank the members of the Ronai and Podbilewicz Labs for discussions. P.W.S. is an Investigator and P.B.G. was an Associate with the HHMI. Support from NCI grant CA59908 (to Z.R.), HFSP, and the Israel Science Foundation (to B.P.) is gratefully acknowledged.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NIHCA59908
Human Frontier Science ProgramUNSPECIFIED
Israel Science FoundationUNSPECIFIED
Subject Keywords:SUMO; somatic gonad; smo-1; lin-11; uterine–vulval connection
Issue or Number:19
PubMed Central ID:PMC522988
Record Number:CaltechAUTHORS:20190514-100517710
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190514-100517710
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95477
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 May 2019 20:57
Last Modified:03 Oct 2019 21:13

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