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Caenorhabditis elegans Gα_q Regulates Egg-Laying Behavior via a PLCβ-Independent and Serotonin-Dependent Signaling Pathway and Likely Functions Both in the Nervous System and in Muscle

Bastiani, Carol A. and Gharib, Shahla and Simon, Melvin I. and Sternberg, Paul W. (2003) Caenorhabditis elegans Gα_q Regulates Egg-Laying Behavior via a PLCβ-Independent and Serotonin-Dependent Signaling Pathway and Likely Functions Both in the Nervous System and in Muscle. Genetics, 165 (4). pp. 1805-1822. ISSN 0016-6731. PMCID PMC1462877. https://resolver.caltech.edu/CaltechAUTHORS:20190514-102529225

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Abstract

egl-30 encodes the single C. elegans ortholog of vertebrate Gα_q family members. We analyzed the expression pattern of EGL-30 and found that it is broadly expressed, with highest expression in the nervous system and in pharyngeal muscle. We isolated dominant, gain-of-function alleles of egl-30 as intragenic revertants of an egl-30reduction-of-function mutation. Using these gain-of-function mutants and existing reduction-of-function mutants, we examined the site and mode of action of EGL-30. On the basis of pharmacological analysis, it has been determined that egl-30 functions both in the nervous system and in the vulval muscles for egg-laying behavior. Genetic epistasis over mutations that eliminate detectable levels of serotonin reveals that egl-30requires serotonin to regulate egg laying. Furthermore, pharmacological response assays strongly suggest that EGL-30 may directly couple to a serotonin receptor to mediate egg laying. We also examined genetic interactions with mutations in the gene that encodes the single C. elegans homolog of PLCβ and mutations in genes that encode signaling molecules downstream of PLCβ. We conclude that PLCβ functions in parallel with egl-30 with respect to egg laying or is not the major effector of EGL-30. In contrast, PLCβ-mediated signaling is likely downstream of EGL-30 with respect to pharyngeal-pumping behavior. Our data indicate that there are multiple signaling pathways downstream of EGL-30 and that different pathways could predominate with respect to the regulation of different behaviors.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://www.genetics.org/content/165/4/1805PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1462877PubMed CentralArticle
ORCID:
AuthorORCID
Sternberg, Paul W.0000-0002-7699-0173
Alternate Title:Caenorhabditis elegans Galphaq regulates egg-laying behavior via a PLCbeta-independent and serotonin-dependent signaling pathway and likely functions both in the nervous system and in muscle
Additional Information:© 2003 by the Genetics Society of America. Manuscript received August 20, 2002; Accepted for publication September 8, 2003. We thank members of the Sternberg and Simon labs for stimulating discussion and review of this manuscript. We also thank the Iwasaki laboratory for providing egl-30(tg26), the Miller laboratory for providing EGL-8 antibody, the Tsien laboratory for providing the RSET vector, Lorna Brundage for providing syIs36 and for injecting pLB24, and the Caenorhabditis Genetics Center for providing strains. This research was supported by the Howard Hughes Medical Institute with which P.W.S. is an investigator and by National Institute of General Medical Science grant no. GM34236 to M.I.S. C.A.B. was supported by a Department of Health and Human Services National Research Service Award (GM18704).
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NIHGM34236
NIHGM18704
Issue or Number:4
PubMed Central ID:PMC1462877
Record Number:CaltechAUTHORS:20190514-102529225
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190514-102529225
Official Citation:Caenorhabditis elegans Gαq Regulates Egg-Laying Behavior via a PLCβ-Independent and Serotonin-Dependent Signaling Pathway and Likely Functions Both in the Nervous System and in Muscle. Carol A. Bastiani, Shahla Gharib, Melvin I. Simon and Paul W. Sternberg. GENETICS December 1, 2003 vol. 165 no. 4 1805-1822
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95479
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 May 2019 20:54
Last Modified:03 Oct 2019 21:13

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