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Control of Vulval Cell Division Number in the Nematode Oscheius/Dolichorhabditis sp. CEW1

Dichtel, Marie-Laure and Louvet-Vallée, Sophie and Viney, Mark E. and Félix, Marie-Anne and Sternberg, Paul W. (2001) Control of Vulval Cell Division Number in the Nematode Oscheius/Dolichorhabditis sp. CEW1. Genetics, 157 (1). pp. 183-197. ISSN 0016-6731. PMCID PMC1461485.

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Spatial patterning of vulval precursor cell fates is achieved through a different two-stage induction mechanism in the nematode Oscheius/Dolichorhabditis sp. CEW1 compared with Caenorhabditis elegans. We therefore performed a genetic screen for vulva mutants in Oscheius sp. CEW1. Most mutants display phenotypes unknown in C. elegans. Here we present the largest mutant category, which affects division number of the vulva precursors P(4-8).p without changing their fate. Among these mutations, some reduce the number of divisions of P4.p and P8.p specifically. Two mutants omit the second cell cycle of all vulval lineages. A large subset of mutants undergo additional rounds of vulval divisions. We also found precocious and retarded heterochronic mutants. Whereas the C. elegans vulval lineage mutants can be interpreted as overall (homeotic) changes in precursor cell fates with concomitant cell cycle changes, the mutants described in Oscheius sp. CEW1 do not affect overall precursor fate and thereby dissociate the genetic mechanisms controlling vulval cell cycle and fate. Laser ablation experiments in these mutants reveal that the two first vulval divisions in Oscheius sp. CEW1 appear to be redundantly controlled by a gonad-independent mechanism and by a gonadal signal that operates partially independently of vulval fate induction.

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Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2001 by the Genetics Society of America. Manuscript received July 24, 2000; Accepted for publication September 18, 2000. We thank Marie Delattre and Ralf Sommer for discussions and reading of the manuscript. We are very grateful to G. Medina, M. Khairy, E. Delattre, and S. Boeuf for preparing the many worm plates that were used. P.W.S. is an Investigator with the Howard Hughes Medical Institute. M.-A.F. was supported during the course of this work by the Howard Hughes Medical Institute and the Centre National de la Recherche Scientifique. M.E.V. was supported by a Medical Research Council Career Development Award. Work in the M.-A.F. laboratory is supported by an ATIPE of Centre National de la Recherche Scientifique, the Fondation pour la Recherche Médicale and the Association pour la Recherche sur le Cancer.
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Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Centre National de la Recherche Scientifique (CNRS)UNSPECIFIED
Medical Research Council (UK)UNSPECIFIED
Fondation pour la Recherche MédicaleUNSPECIFIED
Association pour la Recherche sur le CancerUNSPECIFIED
Issue or Number:1
PubMed Central ID:PMC1461485
Record Number:CaltechAUTHORS:20190514-103856899
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Official Citation:Control of Vulval Cell Division Number in the Nematode Oscheius/Dolichorhabditis sp. CEW1. Marie-Laure Dichtel, Sophie Louvet-Vallée, Mark E. Viney, Marie-Anne Félix and Paul W. Sternberg. GENETICS January 1, 2001 vol. 157 no. 1 183-197
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95481
Deposited By: Tony Diaz
Deposited On:16 May 2019 02:45
Last Modified:03 Oct 2019 21:13

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