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Structural Requirements for the Tissue-Specific and Tissue-General Functions of the Caenorhabditis elegans Epidermal Growth Factor LIN-3

Liu, Jing and Tzou, Phoebe and Hill, Russell J. and Sternberg, Paul W. (1999) Structural Requirements for the Tissue-Specific and Tissue-General Functions of the Caenorhabditis elegans Epidermal Growth Factor LIN-3. Genetics, 153 (3). pp. 1257-1269. ISSN 0016-6731. PMCID PMC1460807. https://resolver.caltech.edu/CaltechAUTHORS:20190514-112200944

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Abstract

Caenorhabditis elegans lin-3 encodes a homolog of the epidermal growth factor (EGF) family of growth factors. LIN-3 is the inductive signal for hermaphrodite vulval differentiation, and it is required for animal viability, hermaphrodite fertility, and the specification of anterior cell fates in the male B cell lineage. We describe the cloning of a lin-3 homolog from C. briggsae, sequence comparison of C. elegans lin-3 with C. briggsae lin-3, and the determination of molecular lesions in alleles of C. elegans lin-3, including three new alleles. We also analyzed the severity of phenotypes caused by the new and existing alleles of lin-3. Correlation of mutant phenotypes and their molecular lesions, as well as sequence comparison between two species, reveal that the EGF motif and the N-terminal portion of the cytoplasmic domain are important for the functions of LIN-3 in all tissues, while the C-terminal portion of the cytoplasmic domain is involved in the tissue-specific functions of lin-3. We discuss how the structure of lin-3 contributes to its functions in multiple developmental processes.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://www.genetics.org/content/153/3/1257.longPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1460807PubMed CentralArticle
ORCID:
AuthorORCID
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 1999 by the Genetics Society of America. Manuscript received August 31, 1998; Accepted for publication July 14, 1999. We thank the Caenorhabditis Genetics Center for providing some of the strains, and T. Clandinin for commenting on the manuscript. This work was supported by a grant from U.S. Public Health Service (HD23690) to P.W.S., who is an Investigator with the Howard Hughes Medical Institute.
Funders:
Funding AgencyGrant Number
NIHHD23690
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:3
PubMed Central ID:PMC1460807
Record Number:CaltechAUTHORS:20190514-112200944
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190514-112200944
Official Citation:Structural Requirements for the Tissue-Specific and Tissue-General Functions of the Caenorhabditis elegans Epidermal Growth Factor LIN-3. Jing Liu, Phoebe Tzou, Russell J. Hill and Paul W. Sternberg. GENETICS November 1, 1999 vol. 153 no. 3 1257-1269
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95487
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 May 2019 20:45
Last Modified:03 Oct 2019 21:13

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