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Chromatographic separation and identification of some peptides in partial hydroylsates of gelatin

Schroeder, W. A. and Honnen, Lewis and Green, F. Charlotte (1953) Chromatographic separation and identification of some peptides in partial hydroylsates of gelatin. Proceedings of the National Academy of Sciences of the United States of America, 39 (1). pp. 23-30. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:SCHRpnas53

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Abstract

Recently we have been engaged in a study of the chemical structure of collagen and gelatin with the object of determining the sequence of the amino acid residues in the polypeptide chains of these proteins. In the course of this study we have made considerable progress in the chromatographic analysis of complex mixtures of peptides and we have isolated and identified several simple peptides which occur in partial hydrolysates of gelatin. The initial separation of the mixture into zones of one or more peptides has been made on a column of ion exchange resin; further separation of the peptides in each zone has been achieved by chromatographing in the form of dinitrophenyl (DNP) peptides on columns of silicic acid-Celite. It is to be hoped that the particular combination of chromatographic methods which has been successfully used in the present study will be helpful in the resolution of the complex mixtures which result from the partial hydrolysis of other proteins.


Item Type:Article
Additional Information:© 1953 by the National Academy of Sciences. Communicated by Linus Pauling, November 4, 1952. This investigation was supported in part by a grant-in-aid from E. I. du Pont de Nemours and Company. Gates and Crellin Laboratories of Chemistry, Contribution No. 1748.
Record Number:CaltechAUTHORS:SCHRpnas53
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:SCHRpnas53
Alternative URL:http://www.pnas.org/cgi/reprint/39/1/23
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9554
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Feb 2008
Last Modified:14 Nov 2014 19:20

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