A Caltech Library Service

Harmonic holography for three-dimensional cellular imaging

Hsieh, Chia-Lung and Grange, Rachel and Pu, Ye and Psaltis, Demetri (2009) Harmonic holography for three-dimensional cellular imaging. In: Three-Dimensional Imaging, Visualization, and Display 2009. Proceedings of SPIE. Vol.7329. Society of Photo-Optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 73290H. ISBN 9780819475954.

[img] PDF - Published Version
See Usage Policy.


Use this Persistent URL to link to this item:


Luminescent markers play a key role in imaging techniques for life sciences since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO_3 nanocrystals. These nanoparticles are attractive due to their stable, non-saturating and coherent signal with a femtosecond-scale response time and a broad flexibility in the choice of excitation wavelength. We use the coherent SHG signal from BaTiO_3 nanoparticles for three-dimensional (3D) imaging without scanning. We built a harmonic holographic (H^2) microscope which records digital holograms at the second harmonic frequency. Highresolution 3D distributions of these SHG markers in mammalian cells are successfully captured and interpreted by the H^2 microscope.

Item Type:Book Section
Related URLs:
URLURL TypeDescription
Additional Information:© 2009 Society of Photo-Optical Instrumentation Engineers (SPIE).
Subject Keywords:second harmonic generation, barium titanate, nanoparticle, digital holography, biological cells, three-dimensional microscopy
Series Name:Proceedings of SPIE
Record Number:CaltechAUTHORS:20190516-111740618
Persistent URL:
Official Citation:Chia-Lung Hsieh, Rachel Grange, Ye Pu, and Demetri Psaltis "Harmonic holography for three-dimensional cellular imaging", Proc. SPIE 7329, Three-Dimensional Imaging, Visualization, and Display 2009, 73290H (6 May 2009); doi: 10.1117/12.820801;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95542
Deposited By: Tony Diaz
Deposited On:16 May 2019 22:11
Last Modified:03 Oct 2019 21:14

Repository Staff Only: item control page