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High viral load in lymph nodes and latent human immunodeficiency virus (HIV) in peripheral blood cells of HIV-1-infected chimpanzees

Saksela, Kalle and Muchmore, Elizabeth and Girard, Marc and Fultz, Patricia and Baltimore, David (1993) High viral load in lymph nodes and latent human immunodeficiency virus (HIV) in peripheral blood cells of HIV-1-infected chimpanzees. Journal of Virology, 67 (12). pp. 7423-7427. ISSN 0022-538X. PMCID PMC238207. https://resolver.caltech.edu/CaltechAUTHORS:SAKjvir93

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Abstract

We have examined human immunodeficiency virus type 1 (HIV-1) infection in chimpanzees by analyzing HIV-1 DNA and RNA in lymph nodes and peripheral mononuclear cells (PBMCs). Like certain asymptomatic HIV-infected persons, these chimpanzees had no detectable viral replication in their PBMCs. However, viral replication and a high viral load were observed in the lymphatic tissue. Despite the absence of viral replication in PBMCs, 1/1,000 to 1/10,000 of the PBMCs contained HIV-1 proviral DNA, and HIV transcription could be rapidly induced in these cells in vitro. These results provide direct evidence of cellular latency of HIV in vivo and suggest that HIV infection in chimpanzees may be a useful model for clinical latency of HIV infection in humans.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://jvi.asm.org/cgi/content/abstract/67/12/7423PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc238207/PubMed CentralArticle
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:© 1993 by the American Society for Microbiology. Received 12 July 1993; Accepted 8 September 1993. We thank Douglas Cohn, Carol Calvano, and the animal technicians at LEMSIP for help in collecting the lymph node and PBMC samples and Marty Scott for helpful comments on the manuscript. K.S. is an Aaron Diamond Foundation postdoctoral research fellow. This study was supported by NIH grant AI22346 to D.B. and by grants from Pasteur Merieux Serums et Vaccins and the French National AIDS Research Agency (ANRS) to M.G.
Funders:
Funding AgencyGrant Number
NIHAI22346
Pasteur Merieux Serums et VaccinsUNSPECIFIED
ANRS (France REcherche Nord&sud Sida-hiv Hépatites)UNSPECIFIED
Issue or Number:12
PubMed Central ID:PMC238207
Record Number:CaltechAUTHORS:SAKjvir93
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:SAKjvir93
Official Citation:High viral load in lymph nodes and latent human immunodeficiency virus (HIV) in peripheral blood cells of HIV-1-infected chimpanzees. K Saksela, E Muchmore, M Girard, P Fultz, D Baltimore Journal of Virology Dec 1993, 67 (12) 7423-7427
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9562
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:04 Feb 2008
Last Modified:28 Apr 2020 16:20

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