CaltechAUTHORS
  A Caltech Library Service

Multistep SlipChip for the Generation of Serial Dilution Nanoliter Arrays and Hepatitis B Viral Load Quantification by Digital Loop Mediated Isothermal Amplification

Yu, Mengchao and Chen, Xiaoying and Qu, Haijun and Ma, Liang and Xu, Lei and Lv, Weiyuan and Wang, Hua and Ismagilov, Rustem F. and Li, Min and Shen, Feng (2019) Multistep SlipChip for the Generation of Serial Dilution Nanoliter Arrays and Hepatitis B Viral Load Quantification by Digital Loop Mediated Isothermal Amplification. Analytical Chemistry, 91 (14). pp. 8751-8755. ISSN 0003-2700. https://resolver.caltech.edu/CaltechAUTHORS:20190523-142111730

[img] PDF - Accepted Version
See Usage Policy.

719Kb
[img] PDF (Methods, measurement of mixing between mother and daughter microwells, bright field images, analysis of fluorescence intensity, sequence of primers used in the LAMP reaction, and author contributions) - Supplemental Material
See Usage Policy.

322Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20190523-142111730

Abstract

Serial dilution is a commonly used technique that generates a low-concentration working sample from a high-concentration stock solution and is used to set up screening conditions over a large dynamic range for biological study, optimization of reaction conditions, drug screening, etc. Creating an array of serial dilutions usually requires cumbersome manual pipetting steps or a robotic liquid handling system. Moreover, it is very challenging to set up an array of serial dilutions in nanoliter volumes in miniaturized assays. Here, a multistep SlipChip microfluidic device is presented for generating serial dilution nanoliter arrays in high throughput with a series of simple sliding motions. The dilution ratio can be precisely predetermined by the volumes of mother microwells and daughter microwells, and this paper demonstrates devices designed to have dilution ratios of 1:1, 1:2, and 1:4. Furthermore, an eight-step serial dilution SlipChip with a dilution ratio of 1:4 is applied for digital loop-mediated isothermal amplification (LAMP) across a large dynamic range and tested for hepatitis B viral load quantification with clinical samples. With 64 wells of each dilution and fewer than 600 wells in total, the serial dilution SlipChip can achieve a theoretical quantification dynamic range of 7 orders of magnitude.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/acs.analchem.9b01270DOIArticle
https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.9b01270/suppl_file/ac9b01270_si_001.pdfPublisherSupporting Information
ORCID:
AuthorORCID
Yu, Mengchao0000-0002-2535-429X
Ismagilov, Rustem F.0000-0002-3680-4399
Shen, Feng0000-0002-4709-330X
Additional Information:© 2019 American Chemical Society. Received: March 11, 2019; Accepted: May 20, 2019. Publication Date: May 22, 2019. This work is supported by the National Natural Science Foundation of China (Grant No. 21705109), the Innovation Research Plan supported by the Shanghai Municipal Education Commission (Grant No. ZXWF082101), and the Shanghai Jiao Tong University Fund (Grant No. WF220408214). The authors declare no competing financial interest.
Funders:
Funding AgencyGrant Number
National Natural Science Foundation of China21705109
Shanghai Municipal Education CommissionZXWF082101
Shanghai Jiao Tong UniversityWF220408214
Issue or Number:14
Record Number:CaltechAUTHORS:20190523-142111730
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190523-142111730
Official Citation:Multistep SlipChip for the Generation of Serial Dilution Nanoliter Arrays and Hepatitis B Viral Load Quantification by Digital Loop Mediated Isothermal Amplification. Mengchao Yu, Xiaoying Chen, Haijun Qu, Liang Ma, Lei Xu, Weiyuan Lv, Hua Wang, Rustem F. Ismagilov, Min Li, and Feng Shen. Analytical Chemistry 2019 91 (14), 8751-8755 DOI: 10.1021/acs.analchem.9b01270
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95766
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 May 2019 21:52
Last Modified:03 Oct 2019 21:16

Repository Staff Only: item control page