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MCH4 is a multicopy suppressor of glycine toxicity in Saccharomyces cerevisiae

Melnykov, Artem V. and Elson, Elliot L. (2019) MCH4 is a multicopy suppressor of glycine toxicity in Saccharomyces cerevisiae. . (Unpublished)

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Saccharomyces cerevisiae can either import amino acids from the surrounding or synthesize inside the cell, and both processes are tightly regulated. Disruption of such regulation can result in amino acid toxicity to the cell through mechanisms that are poorly understood. In this study we make use of a mutant strain with deregulated general amino acid permease gene whose growth is inhibited by low concentrations of several amino acids. We carry out multicopy suppression screen with several toxic amino acids and identify MCH4 as a gene that suppresses inhibitory effects of glycine. We find that expression of MCH4 is regulated by osmotic shock but not other kinds of stress. These findings are discussed in the context of possible mechanisms of amino acid toxicity.

Item Type:Report or Paper (Discussion Paper)
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URLURL TypeDescription Paper
Melnykov, Artem V.0000-0002-4733-4249
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. bioRxiv preprint first posted online May. 29, 2019. Funding was provided by National Institutes of Health (Grant No. 1 R01 HL109505). The authors declare that they have no conflict of interest.
Funding AgencyGrant Number
NIH1 R01 HL109505
Subject Keywords:amino acid toxicity, general amino acid permease, monocarboxylate transporters
Record Number:CaltechAUTHORS:20190529-152315698
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Official Citation:MCH4 is a multicopy suppressor of glycine toxicity in Saccharomyces cerevisiae. Artem V Melnykov, Elliot L Elson. bioRxiv 653444; doi:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:95888
Deposited By: Tony Diaz
Deposited On:29 May 2019 22:51
Last Modified:16 Nov 2021 17:16

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