CaltechAUTHORS
  A Caltech Library Service

How many metals does it take to fix N2? A mechanistic overview of biological nitrogen fixation

Howard, James B. and Rees, Douglas C. (2006) How many metals does it take to fix N2? A mechanistic overview of biological nitrogen fixation. Proceedings of the National Academy of Sciences of the United States of America, 103 (46). pp. 17088-17093. ISSN 0027-8424. PMCID PMC1859894. https://resolver.caltech.edu/CaltechAUTHORS:HOWpnas06

[img]
Preview
PDF - Published Version
See Usage Policy.

1946Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:HOWpnas06

Abstract

During the process of biological nitrogen fixation, the enzyme nitrogenase catalyzes the ATP-dependent reduction of dinitrogen to ammonia. Nitrogenase consists of two component metalloproteins, the iron (Fe) protein and the molybdenum-iron (MoFe) protein; the Fe protein mediates the coupling of ATP hydrolysis to interprotein electron transfer, whereas the active site of the MoFe protein contains the polynuclear FeMo cofactor, a species composed of seven iron atoms, one molybdenum atom, nine sulfur atoms, an interstitial light atom, and one homocitrate molecule. This Perspective provides an overview of biological nitrogen fixation and introduces three contributions to this special feature that address central aspects of the mechanism and assembly of nitrogenase.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.0603978103DOIArticle
http://www.pnas.org/content/103/46/17088.longPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1859894/PubMed CentralArticle
ORCID:
AuthorORCID
Rees, Douglas C.0000-0003-4073-1185
Additional Information:© 2006 by the National Academy of Sciences. Edited by Richard R. Schrock, Massachusetts Institute of Technology, Cambridge, MA, and approved June 28, 2006 (received for review May 19, 2006). Published online on November 6, 2006, 10.1073/pnas.0603978103. We thank members of the Howard and Rees groups, past, present, and future, for their contributions to the nitrogenase project in our laboratories. This work was supported in part by National Institutes of Health Grant GM045162.
Subject Keywords:IRON-MOLYBDENUM COFACTOR; AZOTOBACTER-VINELANDII NITROGENASE; SELECTIVE FE-57 ENRICHMENT; FERROUS FE4S4 CLUSTER; FEMO-COFACTOR; MOFE-PROTEIN; CENTRAL LIGAND; ACTIVE-SITE; CRYSTALLOGRAPHIC STRUCTURE; CLOSTRIDIUM-PASTEURIANUM
Issue or Number:46
PubMed Central ID:PMC1859894
Record Number:CaltechAUTHORS:HOWpnas06
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:HOWpnas06
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9607
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:18 Feb 2008
Last Modified:12 Dec 2019 17:07

Repository Staff Only: item control page