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Activation-dependent changes in receptor distribution and dendritic morphology in hippocampal neurons expressing P2X(2)-green fluorescent protein receptors

Khakh, Baljit S. and Smith, W. Bryan and Chiu, Chi-Sung and Ju, Donghong and Davidson, Norman and Lester, Henry A. (2001) Activation-dependent changes in receptor distribution and dendritic morphology in hippocampal neurons expressing P2X(2)-green fluorescent protein receptors. Proceedings of the National Academy of Sciences of the United States of America, 98 (9). pp. 5288-5293. ISSN 0027-8424. PMCID PMC33202. https://resolver.caltech.edu/CaltechAUTHORS:KHApnas01a

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Abstract

ATP-gated P2X(2) receptors are widely expressed in neurons, but the cellular effects of receptor activation are unclear. We engineered functional green fluorescent protein (GFP)-tagged P2X(2) receptors and expressed them in embryonic hippocampal neurons, and report an approach to determining functional and total receptor pool sizes in living cells. ATP application to dendrites caused receptor redistribution and the formation of varicose hot spots of higher P2X(2)-GFP receptor density. Redistribution in dendrites was accompanied by an activation-dependent enhancement of the ATP-evoked current. Substate-specific mutant T18A P2X(2)-GFP receptors showed no redistribution or activation-dependent enhancement of the ATP-evoked current. Thus fluorescent P2X(2)-GFP receptors function normally, can be quantified, and reveal the dynamics of P2X(2) receptor distribution on the seconds time scale.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.081089198 DOIArticle
http://www.pnas.org/content/98/9/5288.abstractPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC33202/PubMed CentralArticle
ORCID:
AuthorORCID
Lester, Henry A.0000-0002-5470-5255
Additional Information:© 2001 by the National Academy of Sciences. Contributed by Norman Davidson, February 21, 2001. We thank H. Li for preparation of Xenopus oocytes and E. Schuman for advice and use of the confocal microscope. This work was supported by a Wellcome Trust (U.K.) Prize Fellowship (to B.S.K.), by a Roche Bioscience Award, and by the National Institutes of Health (NS-11756, MH 49176). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Errata:For the article “Activation-dependent changes in receptor distribution and dendritic morphology in hippocampal neurons expressing P2X2-green fluorescent protein receptors” by Baljit S. Khakh, W. Bryan Smith, Chi-Sung Chiu, Donghong Ju, Norman Davidson, and Henry A. Lester, which appeared in number 9, April 24, 2001, of Proc. Natl. Acad. Sci. USA (98, 5288–5293; First Published April 10, 2001; 10.1073/pnas.081089198), due to a printer's error, Baljit Khakh's e-mail was incorrectly listed as bsk@mrc-lmb.com.ac.uk. It should be ku.ca.mac.bml-crm@ksb.
Funders:
Funding AgencyGrant Number
Wellcome TrustUNSPECIFIED
Roche BiosciencesUNSPECIFIED
NIHNS-11756
NIHMH 49176
Subject Keywords:ion channel, ATP, filopodia, ionotropic glutamate receptors, single-channel properties, gated cation channels, ion channels, P2X receptor, redistribution, purinoceptors, kinetics, cells, desensitization
Issue or Number:9
PubMed Central ID:PMC33202
Record Number:CaltechAUTHORS:KHApnas01a
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:KHApnas01a
Alternative URL:http://dx.doi.org/10.1073/pnas.081089198
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:961
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:15 Nov 2005
Last Modified:02 Oct 2019 22:38

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