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A flaxseed heteropolysaccharide stimulates immune responses and inhibits hepatitis B virus

Liang, Shan and Li, Xiaofeng and Ma, Xiang and Li, Aijun and Wang, Yong and Reaney, Martin J. T. and Shim, Youn Young (2019) A flaxseed heteropolysaccharide stimulates immune responses and inhibits hepatitis B virus. International Journal of Biological Macromolecules, 136 . pp. 230-240. ISSN 0141-8130. http://resolver.caltech.edu/CaltechAUTHORS:20190612-090229348

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Abstract

A novel heteropolysaccharide defined as FP-1 was enriched from flaxseed hull through hot water extraction (boiling water, 2 h, twice) followed by ion-exchange and size exclusion chromatography. FP-1 is a highly purified heteropolysaccharide with an average molecular weight of 2626 kDa. Its monosaccharide composition includes xylose (35.2%), rhamnose (23.8%), galactose (23.4%), arabinose (10.6%), fucose (4.0%), glucose (2.7%) and mannose (0.3%). Main linkage type of FP-1 backbone includes →2)-α-L-Rhap-(1→, →4)-α-D-GalpA-(1→, →4)-β-D-Xylp-(1→, →3,5)-α-L-Araf-(1→, and →2)-α-D-Xylp-(1→. FP-1 has a triple-helix conformation, indicating its potential bioactivity. FP-1 could stimulate immune responses through inducing mRNA expression of tumor necrosis factor α (TNF-α), nitric oxide (NO), and interleukin (IL-6 and IL-12) in murine macrophages. FP-1 also inhibits hepatitis B virus (HBV) through inhibition of surface antigen (HBsAg) and envelope antigen (HBeAg) expression and interfering with HBV DNA replication. These findings suggest that FP-1 could be potentially developed as functional food ingredient, immune stimulant and vaccine adjuvant.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.ijbiomac.2019.06.076DOIArticle
ORCID:
AuthorORCID
Shim, Youn Young0000-0002-5039-8219
Additional Information:© 2019 Published by Elsevier B.V. Received 19 February 2019, Revised 12 May 2019, Accepted 11 June 2019, Available online 12 June 2019.
Funders:
Funding AgencyGrant Number
National Key R&D Program of China2018YFD0401103
Science and Technology Project of Guangdong Province2017B090907018
Guangdong Province Natural Science Foundation Project2017A030310012
Science and Technology Project of Guangzhou City201803020032
Subject Keywords:Flaxseed heteropolysaccharide; Immunomodulatory; Anti-HBV
Record Number:CaltechAUTHORS:20190612-090229348
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20190612-090229348
Official Citation:Shan Liang, Xiaofeng Li, Xiang Ma, Aijun Li, Yong Wang, Martin J.T. Reaney, Youn Young Shim, A flaxseed heteropolysaccharide stimulates immune responses and inhibits hepatitis B virus, International Journal of Biological Macromolecules, Volume 136, 2019, Pages 230-240, ISSN 0141-8130, https://doi.org/10.1016/j.ijbiomac.2019.06.076. (http://www.sciencedirect.com/science/article/pii/S0141813019312802)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:96315
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:12 Jun 2019 16:36
Last Modified:18 Jun 2019 16:22

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