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The Serotonergic Raphe Promote Sleep in Zebrafish and Mice

Oikonomou, Grigorios and Altermatt, Michael and Zhang, Rong-wei and Coughlin, Gerard M. and Montz, Christin and Gradinaru, Viviana and Prober, David A. (2019) The Serotonergic Raphe Promote Sleep in Zebrafish and Mice. Neuron, 103 (4). pp. 686-701. ISSN 0896-6273. PMCID PMC6706304. http://resolver.caltech.edu/CaltechAUTHORS:20190624-113552525

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Abstract

The role of serotonin (5-HT) in sleep is controversial: early studies suggested a sleep-promoting role, but eventually the paradigm shifted toward a wake-promoting function for the serotonergic raphe. Here, we provide evidence from zebrafish and mice that the raphe are critical for the initiation and maintenance of sleep. In zebrafish, genetic ablation of 5-HT production by the raphe reduces sleep, sleep depth, and the homeostatic response to sleep deprivation. Pharmacological inhibition or ablation of the raphe reduces sleep, while optogenetic stimulation increases sleep. Similarly, in mice, ablation of the raphe increases wakefulness and impairs the homeostatic response to sleep deprivation, whereas tonic optogenetic stimulation at a rate similar to baseline activity induces sleep. Interestingly, burst optogenetic stimulation induces wakefulness in accordance with previously described burst activity of the raphe during arousing stimuli. These results indicate that the serotonergic system promotes sleep in both diurnal zebrafish and nocturnal rodents.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.neuron.2019.05.038DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706304/PublisherArticle
ORCID:
AuthorORCID
Oikonomou, Grigorios0000-0001-6797-7375
Zhang, Rong-wei0000-0003-1145-8589
Gradinaru, Viviana0000-0001-5868-348X
Prober, David A.0000-0002-7371-4675
Additional Information:© 2019 Elsevier Inc. Received 20 September 2018, Revised 11 April 2019, Accepted 22 May 2019, Available online 24 June 2019. Data and Code Availability: The datasets generated and analyzed in this study are available from the corresponding author upon request. We thank the Gradinaru and Prober labs for helpful discussions; Viveca Sapin, Uyen Pham, Hannah Hurley, and Tasha Cammidge for zebrafish husbandry assistance; Chanpreet Singh for generating the Tg(aanat2:ChR2-YFP) line; Elisha D. Mackey for supervising mouse breeding; Andres Collazo for advice on 2-photon laser ablation; Justin S. Bois for advice on statistics; and Catherine M. Oikonomou for critical reading of the manuscript. This work was supported by the NIH (F32NS082010 to G.O.; NIH BRAIN RF1MH117069 to V.G.; and R01NS070911, R01NS101158, R01NS095824, and R01NS101665 to D.A.P.), Center for Molecular and Cellular Neuroscience of the Chen Institute (to V.G.) and the Beckman Institute for CLARITY, Optogenetics and Vector Engineering Research (to V.G.). G.M.C. is supported by a PGS-D from the National Science and Engineering Research Council (NSERC) of Canada. V.G. is a Heritage Principal Investigator supported by the Heritage Medical Research Institute. Author Contributions: G.O., M.A., D.A.P., and V.G. designed experiments. G.O. performed zebrafish experiments. M.A. performed mouse experiments. R.Z. performed zebrafish electrophysiology. G.M.C. performed mouse histology and quantification. C.M. isolated the zebrafish tph2 promoter. V.G. supervised rodent aspects of the project. D.A.P. supervised zebrafish aspects of the project. G.O., M.A., V.G., and D.A.P. wrote the paper with input from R.Z. and G.M.C. The authors declare no competing interests.
Group:Heritage Medical Research Institute, Tianqiao and Chrissy Chen Institute for Neuroscience
Funders:
Funding AgencyGrant Number
NIH Postdoctoral FellowshipF32NS082010
NIHRF1MH117069
NIHR01NS070911
NIHR01NS101158
NIHR01NS095824
NIHR01NS101665
Tianqiao and Chrissy Chen Institute for NeuroscienceUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
Heritage Medical Research InstituteUNSPECIFIED
Subject Keywords:sleep; arousal; serotonin; 5-HT; raphe; optogenetics; fiber photometry
Issue or Number:4
PubMed Central ID:PMC6706304
Record Number:CaltechAUTHORS:20190624-113552525
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20190624-113552525
Official Citation:Grigorios Oikonomou, Michael Altermatt, Rong-wei Zhang, Gerard M. Coughlin, Christin Montz, Viviana Gradinaru, David A. Prober, The Serotonergic Raphe Promote Sleep in Zebrafish and Mice, Neuron, Volume 103, Issue 4, 2019, Pages 686-701.e8, ISSN 0896-6273, https://doi.org/10.1016/j.neuron.2019.05.038. (http://www.sciencedirect.com/science/article/pii/S089662731930491X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:96666
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Jun 2019 18:46
Last Modified:26 Aug 2019 22:34

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