Rice, Douglas and Baltimore, David (1982) Regulated expression of an immunoglobulin kappa gene introduced into a mouse lymphoid cell line. Proceedings of the National Academy of Sciences of the United States of America, 79 (24). pp. 7862-7865. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:RICpnas82
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Abstract
We have introduced a functionally rearranged murine ĸ light chain immunoglobulin (Ig) gene into an Abelson murine leukemia virus-transformed lymphoid cell line. Plasmid pSV2gpt-κ41, containing the ĸ light chain gene from the myeloma MOPC41 and the selectable marker gene gpt, was introduced into 81A-2 cells by the calcium phosphate coprecipitation technique. Cells expressing the gpt gene were selected by growth in medium containing mycophenolic acid. One transfected cell line, ĸ-2, was shown to make ĸ mRNA and polypeptide chains and to assemble the ĸ chain product with γ2b heavy chains to form an apparently complete IgG2b. When bacterial lipopolysaccharide was added to the growth medium, levels of mRNA and polypeptide increased, showing regulated expression of the introduced ĸ gene.
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| Additional Information: | © 1982 by the National Academy of Sciences. Contributed by David Baltimore, September 16, 1982. We thank Drs. F. Alt, M. Boss, S. Lewis, and R. Mulligan for helpful discussions. We thank Dr. R. Mulligan for plasmid pSV2gpt and Dr. P. Leder for the cloned MOPC41 ĸ gene. This work was supported by Grant MV-34N from the American Cancer Society, Grant CA14051 (core grant to S.E. Luria) from the National Cancer Institute, and a contribution from the Whitehead Charitable Foundation. D.R. was supported by a Helen Hay Whitney Postdoctoral Fellowship. D.B. is an American Cancer Society Research Professor. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact. | ||||
| Subject Keywords: | Abelson murine leukemia virus; DNA transfection; gpt selection; lipopolysaccharide induction | ||||
| Issue or Number: | 24 | ||||
| Record Number: | CaltechAUTHORS:RICpnas82 | ||||
| Persistent URL: | http://resolver.caltech.edu/CaltechAUTHORS:RICpnas82 | ||||
| Alternative URL: | http://dx.doi.org/10.1073/pnas.79.24.7862 | ||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||
| ID Code: | 9671 | ||||
| Collection: | CaltechAUTHORS | ||||
| Deposited By: | Tony Diaz | ||||
| Deposited On: | 27 Feb 2008 | ||||
| Last Modified: | 31 Oct 2017 18:11 |
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