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Primary wall cellulose synthase regulates shoot apical meristem mechanics and growth

Sampathkumar, Arun and Peaucelle, Alexis and Fujita, Miki and Schuster, Christoph and Persson, Staffan and Wasteneys, Geoffrey O. and Meyerowitz, Elliot M. (2019) Primary wall cellulose synthase regulates shoot apical meristem mechanics and growth. Development, 146 (10). Art. No. dev179036. ISSN 0950-1991. PMCID PMC6550022. doi:10.1242/dev.179036. https://resolver.caltech.edu/CaltechAUTHORS:20190627-082926521

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Abstract

How organisms attain their specific shapes and modify their growth patterns in response to environmental and chemical signals has been the subject of many investigations. Plant cells are at high turgor pressure and are surrounded by a rigid yet flexible cell wall, which is the primary determinant of plant growth and morphogenesis. Cellulose microfibrils, synthesized by plasma membrane-localized cellulose synthase complexes, are major tension-bearing components of the cell wall that mediate directional growth. Despite advances in understanding the genetic and biophysical regulation of morphogenesis, direct studies of cellulose biosynthesis and its impact on morphogenesis of different cell and tissue types are largely lacking. In this study, we took advantage of mutants of three primary cellulose synthase (CESA) genes that are involved in primary wall cellulose synthesis. Using field emission scanning electron microscopy, live cell imaging and biophysical measurements, we aimed to understand how the primary wall CESA complex acts during shoot apical meristem development. Our results indicate that cellulose biosynthesis impacts the mechanics and growth of the shoot apical meristem.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1242/dev.179036DOIArticle
https://dev.biologists.org/content/146/10/dev179036.supplementalPublisherSupplementary Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6550022/PubMed CentralArticle
ORCID:
AuthorORCID
Sampathkumar, Arun0000-0003-1703-0137
Peaucelle, Alexis0000-0002-8582-6500
Schuster, Christoph0000-0002-1948-2367
Persson, Staffan0000-0002-6377-5132
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2019 Published by The Company of Biologists Ltd. Received April 5, 2019; Accepted May 2, 2019. We thank Olivier Hamant for critical reading and comments on the manuscript; René Schneider for suggesting the use of PlotsofData for generation of violin plots; Paul Tarr and all members of the Meyerowitz lab at the California Institute of Technology for helpful discussions on the topic. We also thank the University of British Columbia Bioimaging Facility for access to the scanning electron microscope. The authors declare no competing or financial interests. Author contributions: Conceptualization: A.S., E.M.M.; Methodology: A.S., A.P., M.F., C.S., G.O.W.; Validation: A.S., M.F., C.S.; Formal analysis: A.S., A.P., M.F., C.S., S.P., G.O.W., E.M.M.; Investigation: A.S., A.P., M.F., C.S., S.P., G.O.W., E.M.M.; Resources: A.S., S.P., G.O.W., E.M.M.; Data curation: A.S., A.P., M.F., C.S.; Writing - original draft: A.S.; Writing - review & editing: A.S., C.S., S.P., G.O.W., E.M.M.; Visualization: A.S., A.P., M.F.; Supervision: G.O.W., E.M.M.; Funding acquisition: A.S., E.M.M. A.S. is supported by the Max-Planck-Gesellschaft. C.S. is supported by a Gatsby Charitable Foundation fellowship (GAT3395/DAA). S.P. was supported by an ARC Future Fellowship grant (FT160100218). G.O.W. was supported by a Natural Sciences and Engineering Research Council of Canada Discovery Grant (2014-06080). E.M.M. is a Howard Hughes Medical Institute investigator. Deposited in PMC for release after 12 months.
Funders:
Funding AgencyGrant Number
Max-Planck-GesellschaftUNSPECIFIED
Gatsby Charitable FoundationGAT3395/DAA
Australian Research CouncilFT160100218
Natural Sciences and Engineering Research Council of Canada (NSERC)2014-06080
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:Cell wall, Cell growth, Cellulose, Mechanics, Microtubules, Morphogenesis
Issue or Number:10
PubMed Central ID:PMC6550022
DOI:10.1242/dev.179036
Record Number:CaltechAUTHORS:20190627-082926521
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190627-082926521
Official Citation:Primary wall cellulose synthase regulates shoot apical meristem mechanics and growth. Arun Sampathkumar, Alexis Peaucelle, Miki Fujita, Christoph Schuster, Staffan Persson, Geoffrey O. Wasteneys, Elliot M. Meyerowitz. Development 2019 146: dev179036 doi: 10.1242/dev.179036 Published 24 May 2019
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:96764
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:27 Jun 2019 16:17
Last Modified:16 Nov 2021 17:23

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