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Gene localization by chromosome fractionation: Globin genes are on at least two chromosomes and three estrogen-inducible genes are on three chromosomes

Hughes, Stephen H. and Stubblefield, Elton and Payvar, Farhang and Engel, James D. and Dodgson, Jerry B. and Spector, Deborah and Cordell, Barbara and Schimke, Robert T. and Varmus, Harold E. (1979) Gene localization by chromosome fractionation: Globin genes are on at least two chromosomes and three estrogen-inducible genes are on three chromosomes. Proceedings of the National Academy of Sciences of the United States of America, 76 (3). pp. 1348-1352. ISSN 0027-8424.

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Chicken metaphase chromosomes were partially purified by rate zonal centrifugation, and DNA was prepared from each of the fractions of the sucrose gradient. The DNA was digested with various restriction enzymes and subjected to electrophoresis in agarose gels. The DNA was transferred to nitrocellulose filters (as described by Southern), and the filters were hybridized with cDNA probes. Four globin genes αA, αD, ß, and ρ or ε are located on at least two chromosomes, and three of the estrogen-inducible genes of the hen oviduct-ovalbumin, ovomucoid, and transferrin--are on three different chromosomes. These experiments also confirm our earlier assignment of the endogenous viral sequence related to Rous-associated virus-0 to a separate (and larger) chromosome than the cellular sequence related to the transforming gene of avian sarcoma virus (cellular sarc), although it now appears that cellular sarc is on a small macrochromosome, rather than on a microchromosome.

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Additional Information:© 1979 by the National Academy of Sciences. Contributed by Robert T. Schimke, November 27, 1978. We are grateful to Dr. John Allred, Department of Biophysics, University of Houston, for allowing the use of his FMF equipment and to Mr. James Oro for technical assistance in its operation. This work was supported in part by Research Grant PCM75-05622 from the National Science Foundation (to F.S.), Research Grants NP 148 from the American Cancer Society and 14931 from the National Institute of General Medical Sciences (to R.T.S.), and American Cancer Society Grant VC-70 (to H.E.V.). S.H.H. was a fellow of the Leukemia Society of America; J.D.E. and D.S. were Helen Hay Whitney Fellows; and J.B.D. was a Fellow of the American Cancer Society. The publication costs of this article were defrayed in part by page charge payments. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:chromosome isolation; gene mapping; flow microfluorimetry; endogenous viral genes
Issue or Number:3
Record Number:CaltechAUTHORS:HUGpnas79
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9694
Deposited By: Tony Diaz
Deposited On:04 Mar 2008
Last Modified:03 Oct 2019 00:02

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