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Efficiency of polymerase chain reaction processes: A stochastic model

Hassibi, Arjang and Sharif, Masoud (2006) Efficiency of polymerase chain reaction processes: A stochastic model. In: IEEE International Workshop on Genomic Signal Processing and Statistics, 2006 (GENSIPS '06), College Station, TX, 28-30 May 2006. IEEE , Piscataway, NJ, pp. 35-36. ISBN 1-4244-0385-5.

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A stochastic model for the efficiency of polymerase chain reaction (PCR) processes is presented. The model is based on the assumption that the i’th nucleotide incorporation on the DNA template takes τi seconds, where τi has an exponential distribution with mean 1/λi. In this paper, given λi that can be obtained from the primer-template sequence, temperature profile, enzyme rate, and other assay conditions [3], we calculate the efficiency of a multi-step PCR process using the distribution of the summation of non-identical exponential distributions.

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Additional Information:© Copyright 2006 IEEE. Reprinted with permission. Publication Date: May 2006. Posted online: 2007-04-30.
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ID Code:9697
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Deposited On:05 Mar 2008
Last Modified:08 Nov 2021 21:01

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