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Coordination of Tissue Cell Polarity by Auxin Transport and Signaling

Verna, Carla and Ravichandran, Sree Janani and Sawchuk, Megan G. and Linh, Nguyen Manh and Scarpella, Enrico (2019) Coordination of Tissue Cell Polarity by Auxin Transport and Signaling. eLife, 8 . Art. No. e51061. ISSN 2050-084X. PMCID PMC6890459. https://resolver.caltech.edu/CaltechAUTHORS:20190716-120637201

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Abstract

Plants coordinate the polarity of hundreds of cells during vein formation, but how they do so is unclear. The prevailing hypothesis proposes that GNOM, a regulator of membrane trafficking, positions PIN-FORMED auxin transporters to the correct side of the plasma membrane; the resulting cell-to-cell, polar transport of auxin would coordinate tissue cell polarity and induce vein formation. Contrary to predictions of the hypothesis, we find that vein formation occurs in the absence of PIN-FORMED or any other intercellular auxin-transporter; that the residual auxin-transport-independent vein-patterning activity relies on auxin signaling; and that a GNOM-dependent signal acts upstream of both auxin transport and signaling to coordinate tissue cell polarity and induce vein formation. Our results reveal synergism between auxin transport and signaling, and their unsuspected control by GNOM in the coordination of tissue cell polarity during vein patterning, one of the most informative expressions of tissue cell polarization in plants.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.7554/eLife.51061DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6890459/PubMed CentralArticle
https://doi.org/10.1101/680090DOIDiscussion Paper
ORCID:
AuthorORCID
Verna, Carla0000-0001-5312-5470
Scarpella, Enrico0000-0003-2962-0329
Additional Information:© 2019, Verna et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. Received: August 13, 2019. Accepted: November 1, 2019. Version of Record published: December 3, 2019 (version 1) We dedicate this article to Ida Ruberti, an inspiring example of scientific depth, integrity, and elegance; Ida passed away on June 8, 2019. We thank the Arabidopsis Biological Resource Center for emb30-8, gn-13, gn-18, pin1-1, eir1-1, pin6, pin8-1, pgp1-100, mdr1-101, ucu2-4, aux1-355, lax1-064, axr1-3 and axr1-12; Hidehiro Fukaki for fwr and gnSALK_103014; Sandra Richter and Gerd Jürgens for gnB/E and gnR5; Satoshi Naramoto and Hiroo Fukuda for van7/emb30-7; Eva Benková and Jiří Friml for PIN1::PIN1:GFP; Jian Xu and Ben Scheres for PIN1::PIN1:YFP and PIN2::PIN2:GFP; Jian Xu, Miyo Morita, and Masao Tasaka for PIN3::PIN3:GFP; Ikram Blilou and Ben Scheres for Atpin1:: En134, pin3-3, pin4-2 and pin7En; Venkatesan Sundaresan for toz-1; Thomas Berleth for mpG12; Markus Geisler and Jiří Friml for ABCB1::ABCB1:GFP and ABCB19::ABCB19:GFP; Cris Kuhlemeier for aux1-21;lax1;2–1;3; Michael Prigge and Mark Estelle for tir1-1;afb2-3; and Marcus Heisler and Elliot Meyerowitz for DR5rev::nYFP. This work was supported by Discovery Grants of the Natural Sciences and Engineering Research Council of Canada (NSERC) to ES. CV was supported, in part, by a University of Alberta Doctoral Recruitment Scholarship. MGS was supported, in part, by an NSERC CGS-M Scholarship and an NSERC CGS-D Scholarship. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Author contributions: Carla Verna, Sree Janani Ravichandran, Megan G Sawchuk, Nguyen Manh Linh, Conceptualization, Formal analysis, Validation, Investigation, Visualization, Methodology, Writing—original draft, Writing—review and editing; Enrico Scarpella, Conceptualization, Resources, Formal analysis, Supervision, Funding acquisition, Validation, Investigation, Visualization, Methodology, Writing—original draft, Project administration, Writing—review and editing. Data availability: All data generated or analyzed during this study are included in the manuscript and supporting files.
Funders:
Funding AgencyGrant Number
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
University of AlbertaUNSPECIFIED
PubMed Central ID:PMC6890459
Record Number:CaltechAUTHORS:20190716-120637201
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190716-120637201
Official Citation:Verna et al. eLife 2019;8:e51061. DOI: https://doi.org/10.7554/eLife.51061
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:97176
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Jul 2019 21:54
Last Modified:21 Dec 2019 16:50

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