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Coordination of Tissue Cell Polarity by Auxin Transport and Signaling

Verna, Carla and Ravichandran, Sree Janani and Sawchuk, Megan G. and Linh, Nguyen Manh and Scarpella, Enrico (2019) Coordination of Tissue Cell Polarity by Auxin Transport and Signaling. . (Unpublished)

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Coordination of polarity between cells in tissues is key to multicellular organism development. In animals, coordination of this tissue cell polarity often requires direct cellcell interactions and cell movements, which are precluded in plants by a wall that separates cells and holds them in place; yet plants coordinate the polarity of hundreds of cells during the formation of the veins in their leaves. Overwhelming experimental evidence suggests that the plant signaling molecule auxin coordinates tissue cell polarity to induce vein formation, but how auxin does so is unclear. The prevailing hypothesis proposes that GNOM, a regulator of vesicle formation during protein trafficking, positions auxin transporters of the PIN-FORMED family to the correct side of the plasma membrane. The resulting cell-to-cell, polar transport of auxin would coordinate cell polarity and would induce vein formation. Here we tested this hypothesis by means of a combination of cellular imaging, molecular genetic analysis, and chemical induction and inhibition. Contrary to predictions of the hypothesis, we find that auxin-induced vein formation occurs in the absence of PIN-FORMED proteins or any known intercellular auxin transporter; that the auxin-transport-independent vein-patterning activity relies on auxin signaling; and that a GNOM-dependent signal that coordinates tissue cell polarity to induce vein formation acts upstream of both auxin transport and signaling. Our results reveal a synergism between auxin transport and signaling and their unsuspected control by GNOM in the coordination of cell polarity during vein patterning, one of the most spectacular and informative expressions of tissue cell polarization in plants.

Item Type:Report or Paper (Discussion Paper)
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Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission. bioRxiv preprint first posted online Jul. 11, 2019. We thank the Arabidopsis Biological Resource Center for emb30-8, gn-13, pin1-1, eir1-1, pin6, pin8-1, pgp1-100, mdr1-101, ucu2-4, aux1-355, lax1-064, axr1-3 and axr1-12; Hidehiro Fukaki for fwr and gn^(SALK_103014); Sandra Richter and Gerd Jurgens for gnB/E and gnR5; Satoshi Naramoto and Hiroo Fukuda for van7/emb30-7; Eva Benková and Jirí Friml for PIN1::PIN1:GFP; Jian Xu and Ben Scheres for PIN1::PIN1:YFP and PIN2::PIN2:GFP; Jian Xu, Miyo Morita and Masao Tasaka for PIN3::PIN3:GFP; Ikram Blilou and Ben Scheres for Atpin1::En134, pin3-3, pin4-2 and pin7^(En); Venkatesan Sundaresan for toz-1, Thomas Berleth for mp^(G1)2; Markus Geisler and Jirí Friml for ABCB1::ABCB1:GFP and ABCB19::ABCB19:GFP; Cris Kuhlemeier for aux1-21;lax1;2-1;3; Michael Prigge and Mark Estelle for tir1-1;afb2-3; and Marcus Heisler and Elliot Meyerowitz for DR5rev::nYFP. This work was supported by Discovery Grants of the Natural Sciences and Engineering Research Council of Canada (NSERC) to E.S. C.V. was supported, in part, by a University of Alberta Doctoral Recruitment Scholarship. MGS was supported, in part, by an NSERC CGS-M Scholarship and an NSERC CGS-D Scholarship.
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Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
University of AlbertaUNSPECIFIED
Record Number:CaltechAUTHORS:20190716-120637201
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Official Citation:Coordination of Tissue Cell Polarity by Auxin Transport and Signaling. Carla Verna, Sree Janani Ravichandran, Megan G. Sawchuk, Nguyen Manh Linh, Enrico Scarpella. bioRxiv 680090; doi:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:97176
Deposited By: Tony Diaz
Deposited On:16 Jul 2019 21:54
Last Modified:09 Aug 2019 18:23

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