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A Universal HPLC-MS Method to Determine the Stereochemistry of Common and Unusual Amino Acids

Hess, Sonja (2019) A Universal HPLC-MS Method to Determine the Stereochemistry of Common and Unusual Amino Acids. In: Amino Acid Analysis: Methods and Protocols. Methods in Molecular Biology. No.2030. Humana Press , New York, NY, pp. 263-275. ISBN 978-1-4939-9638-4. https://resolver.caltech.edu/CaltechAUTHORS:20190729-092358870

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Abstract

The determination of the stereochemistry of common and unusual amino acids is important in food chemistry, archeology, medicine, and life sciences including such diverse areas as marine biology and extraterrestrial chemistry and has greatly contributed to our current knowledge in these fields. To determine the stereochemistry of amino acids, many chromatographic methods have been developed and refined over the last decades. Here we describe a state-of-the-art indirect chromatography-based LC-MS method. Diastereomers were formed from amino acids that were reacted with chiral derivatizing agents such as Marfey’s reagent (FDAA), GITC, S-NIFE, and OPA-IBLC and separated on a reversed phase column using mass spectrometry compatible buffers.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1007/978-1-4939-9639-1_20DOIArticle
ORCID:
AuthorORCID
Hess, Sonja0000-0002-5904-9816
Additional Information:© 2019 Springer Science+Business Media, LLC, part of Springer Nature. First Online: 26 July 2019. This work was supported by the Intramural Program of the National Institute of Diabetes, Digestive and Kidney Diseases, Z01 DK070004-04, the Beckman Institute, and the Betty and Gordon Moore Foundation.
Funders:
Funding AgencyGrant Number
NIHZ01 DK070004-04
National Institute of Diabetes and Digestive and Kidney DiseasesUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Subject Keywords:Amino acid derivatization; Enantiomer; Marfey’s reagent; FDAA; GITC; S-NIFE; OPA-IBLC; Amino acid analysis
Series Name:Methods in Molecular Biology
Issue or Number:2030
Record Number:CaltechAUTHORS:20190729-092358870
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190729-092358870
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:97481
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:29 Jul 2019 16:58
Last Modified:03 Oct 2019 21:32

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