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Stimulated Raman imaging with chemical probes for subcellular bioanalysis

Wei, Lu (2019) Stimulated Raman imaging with chemical probes for subcellular bioanalysis. In: 258th ACS National Meeting & Exposition, 25-29 August 2019, San Diego, CA.

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Innovations in optical spectroscopy and microscopy have revolutionized our understanding in biol. systems at subcellular levels. In this talk, I will present recent advances of two chem. imaging strategies addressing two fundamental challenges in optical bio-imaging. First, we devised a live-cell Bioorthogonal Chem. Imaging platform suited for probing the metabolic dynamics of small bio-mols., which cannot be effectively labeled by bulky fluorophores. This scheme couples the stimulated Raman scattering (SRS) microscopy, a nonlinear vibrational imaging modality that offers rich chem. information, with small vibrational tags including stable isotopes and triple bonds. We applied this platform to a variety of dynamical biol. systems and revealed previously less-known heterogeneity in metabolic signatures governing the obversed cell phenotypes. Second, we developed a supermultiplex optical imaging technique enabled by preresonant SRS microscopy of a newly designed palette of vibrational dyes. This work allows for simultaneous imaging of a large no. of mol. species in live cells and tissues with high sensitivity and specificity, bridging imaging and omics paradigms. Both phys. and chem. principles underlying the optical microscopy will be presented, as well as our recent efforts in biomedical applications including cancer- and neuronal- metab.

Item Type:Conference or Workshop Item (Paper)
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Wei, Lu0000-0001-9170-2283
Additional Information:© 2019 American Chemical Society.
Record Number:CaltechAUTHORS:20190812-141405591
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:97792
Deposited By: Tony Diaz
Deposited On:12 Aug 2019 21:25
Last Modified:23 Oct 2019 20:58

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