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A Direct Interaction of Axonin-1 with NgCAM-related Cell Adhesion Molecule (NrCAM) Results in Guidance, but not Growth of Commissural Axons

Fitzli, Dora and Stoeckli, Esther T. and Kunz, Stefan and Siribour, Kingsley and Rader, Christoph and Kunz, Beat and Kozlov, Serguei V. and Buchstaller, Andrea and Lane, Robert P. and Suter, Daniel M. and Dreyer, William J. and Sonderegger, Peter (2000) A Direct Interaction of Axonin-1 with NgCAM-related Cell Adhesion Molecule (NrCAM) Results in Guidance, but not Growth of Commissural Axons. Journal of Cell Biology, 149 (4). pp. 951-968. ISSN 0021-9525. PMCID PMC2174557. doi:10.1083/jcb.149.4.951.

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An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elongation. In an in vitro assay, commissural axons grew preferentially on stripes coated with a mixture of NrCAM and NgCAM. This preference was abolished in the presence of anti–axonin-1 antibodies without a decrease in neurite length. Consistent with these findings, commissural axons in vivo only fail to extend along the longitudinal axis when both NrCAM and NgCAM interactions, but not when axonin-1 and NrCAM or axonin-1 and NgCAM interactions, are perturbed. Thus, we conclude that axonin-1 is involved in guidance of commissural axons without promoting their growth.

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Additional Information:© The Rockefeller University Press, 2000. Submitted: 20 December 1999. Revised: 22 March 2000. Accepted: 7 April 2000. We thank Ulrike Binkle and Claudia A.O. Stuermer for providing matrices for the stripe assay, Christine Mirzayan and Marc Tessier-Lavigne for providing the netrin-1–expressing cell line. We are indebted to Vance Lemmon for many stimulating discussions and helpful technical advice. We thank Ned Mantei for critical reading of the manuscript and Martin Zerner for help with statistics. This work was supported by grants of the Swiss National Science Foundation and the Biotechnology Programme of the European Union.
Funding AgencyGrant Number
Swiss National Science Foundation (SNSF)UNSPECIFIED
Subject Keywords:axon guidance, growth cone, neuronal cell adhesion molecules, immunoglobulin superfamily, signal transduction
Issue or Number:4
PubMed Central ID:PMC2174557
Record Number:CaltechAUTHORS:FITjcb00
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9786
Deposited By: Archive Administrator
Deposited On:16 Mar 2008
Last Modified:08 Nov 2021 21:02

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