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Developmentally regulated expression of a truncated myosin light-chain 1F/3F gene

Garfinkel, Leonard I. and Davidson, Norman (1987) Developmentally regulated expression of a truncated myosin light-chain 1F/3F gene. Molecular and Cellular Biology, 7 (10). pp. 3826-3829. ISSN 0270-7306.

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Fast skeletal muscle myosin light-chain I (MLC1f) and myosin light-chain 3 (MLC3f) mRNAs are both derived from a single rat MLC1/3f gene. MLC1f mRNA begins at the first exon of the gene, while MLC3f mRNA begins with exon 2, 10 kilobases downstream. Both mRNAs require alternate splicing of internal exons for accurate expression. We showed that a truncated rat MLC1f/3f gene lacking exon 1 and the first 6.3 kilobases of the intron separating exons 1 and 2 produced rat MLC3f mRNA in a developmentally regulated manner after introduction into myogenic mouse cells, thus demonstrating in vivo the presence of a functional promoter associated with exon 2. Correctly spliced mRNA was produced after transfer of this truncated gene into both myogenic and nonmyogenic cells, indicating that the pattern of splicing of this complex transcript was due to a structural features of the RNA and was independent of cell type.

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Additional Information:Copyright © 1987 by the American Society for Microbiology. Received 9 March 1987/Accepted 23 July 1987 We acknowledge the expert technical assistance of Marie Krempin. We also thank Carlos Gitler of the Department of Membrane Research, Weizmann Institute of Science, in whose laboratory part of this work was done. This research was supported by a grant from the Muscular Dystrophy Association to N.D. L.I.G. was supported by National Research Service Award 5F32GM09157 from the National Institutes of Health.
Issue or Number:10
Record Number:CaltechAUTHORS:GARmcb87
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9787
Deposited By: Archive Administrator
Deposited On:17 Mar 2008
Last Modified:03 Oct 2019 00:03

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