A Caltech Library Service

Co-translational protein targeting by the signal recognition particle

Shan, Shu-ou and Walter, Peter (2005) Co-translational protein targeting by the signal recognition particle. FEBS Letters, 579 (4). pp. 921-926. ISSN 0014-5793. doi:10.1016/j.febslet.2004.11.049.

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item:


The signal recognition particle (SRP) mediates the co‐translational targeting of nascent proteins to the eukaryotic endoplasmic reticulum membrane, or the bacterial plasma membrane. During this process, two GTPases, one in the SRP and one in the SRP receptor (SR), form a complex in which both proteins reciprocally activate the GTPase reaction of one another. The recent crystal structures of the T. aquaticus SRP · SR complex show that the two GTPases associate via an unusually extensive and highly cooperative interaction surface, and form a composite active site at the interface. GTPase activation proceeds through a unique mechanism, stimulated by both interactions between the twinned GTP molecules across the dimer interface and by conformational rearrangements that position catalytic residues in each active site with respect to the bound substrates. Distinct classes of mutations have been isolated that inhibit specific stages during SRP–SR complex formation and activation, suggesting discrete conformational stages during formation of the active SRP · SR complex. Each stage provides a potential control point in the targeting reaction at which regulation by additional components can be exerted, thus ensuring the binding and release of cargo at the appropriate time.

Item Type:Article
Related URLs:
URLURL TypeDescription
Shan, Shu-ou0000-0002-6526-1733
Additional Information:© 2005 Federation of European Biochemical Societies. Issue Online: 28 November 2004; Version of Record online: 28 November 2004; Manuscript accepted: 22 November 2004; Manuscript revised: 02 November 2004; Manuscript received: 02 November 2004.
Issue or Number:4
Record Number:CaltechAUTHORS:20190814-113039620
Persistent URL:
Official Citation:Shan, Shu-ou and Walter, Peter (2005), Co-translational protein targeting by the signal recognition particle, FEBS Letters, 579, doi: 10.1016/j.febslet.2004.11.049
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:97890
Deposited By: Tony Diaz
Deposited On:15 Aug 2019 17:45
Last Modified:16 Nov 2021 17:35

Repository Staff Only: item control page