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Hydrogen bonding in enzymatic catalysis: Analysis of energetic contributions

Shan, Shu-ou and Herschlag, Daniel (1999) Hydrogen bonding in enzymatic catalysis: Analysis of energetic contributions. In: Enzyme kinetics and mechanism Part E: Energetics of Enzyme Catalysis. Methods in Enzymology. No.308. Academic Press , New York, NY, pp. 246-276. ISBN 9780121822095.

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Enzymes typically form hydrogen bonds with substrate moieties that undergo electronic rearrangement in the course of reaction. This article reviews results that suggest that these hydrogen bonds can be strengthened in the transition state to a greater extent within the context of the enzymatic active site than in aqueous solution. Comparisons in model systems demonstrate this differential strengthening effect and suggest that it may provide substantial rate enhancements for enzymatic reactions relative to solution reactions. Despite their likely importance, quantitative dissection of the role of these "reaction center" hydrogen bonds in enzymatic catalysis is blurred because the energetic contributions of these hydrogen bonds are intertwined with those of hydrogen bonds and other interactions that fold the enzyme and position the bound substrate. This interconnection between binding interactions and rate enhancement is a fundamental property of enzymatic catalysis.

Item Type:Book Section
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Shan, Shu-ou0000-0002-6526-1733
Additional Information:© 1999 by Academic Press. We thank J. Brauman and G. Nadikar for stimulating discussions, I. Petrounia and R. M. Pollack for communicating unpublished results, and the Herschlag laboratory for comments on the manuscript.
Series Name:Methods in Enzymology
Issue or Number:308
Record Number:CaltechAUTHORS:20190814-142341729
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Official Citation:Shu-ou Shan, Daniel Herschlag, Hydrogen bonding in enzymatic catalysis: Analysis of energetic contributions, Methods in Enzymology, Academic Press, Volume 308, 1999, Pages 246-276, ISSN 0076-6879, ISBN 9780121822095, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:97895
Deposited By: Tony Diaz
Deposited On:15 Aug 2019 17:37
Last Modified:16 Nov 2021 17:35

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