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Acoustic biomolecules enhance hemodynamic functional ultrasound imaging of neural activity

Maresca, David and Payen, Thomas and Lee-Gosselin, Audrey and Ling, Bill and Malounda, Dina and Demené, Charlie and Tanter, Mickaël and Shapiro, Mikhail G. (2020) Acoustic biomolecules enhance hemodynamic functional ultrasound imaging of neural activity. NeuroImage, 209 . Art. No. 116467. ISSN 1053-8119. PMCID PMC6955150. https://resolver.caltech.edu/CaltechAUTHORS:20190815-094537072

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Abstract

Hemodynamic functional ultrasound imaging (fUS) of neural activity provides a unique combination of spatial coverage, spatiotemporal resolution and compatibility with freely moving animals. However, deep and transcranial monitoring of brain activity and the imaging of dynamics in slow-flowing blood vessels remains challenging. To enhance fUS capabilities, we introduce biomolecular hemodynamic enhancers based on gas vesicles (GVs), genetically encodable ultrasound contrast agents derived from buoyant photosynthetic microorganisms. We show that intravenously infused GVs enhance ultrafast Doppler ultrasound contrast and visually-evoked hemodynamic contrast in transcranial fUS of the mouse brain. This hemodynamic contrast enhancement is smoother than that provided by conventional microbubbles, allowing GVs to more reliably amplify neuroimaging signals.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.neuroimage.2019.116467DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6955150/PubMed CentralArticle
https://doi.org/10.1101/734939DOIDiscussion Paper
ORCID:
AuthorORCID
Maresca, David0000-0002-4921-6406
Lee-Gosselin, Audrey0000-0002-2431-2741
Malounda, Dina0000-0001-7086-9877
Demené, Charlie0000-0002-5329-700X
Tanter, Mickaël0000-0001-7739-8051
Shapiro, Mikhail G.0000-0002-0291-4215
Additional Information:© 2019 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Received 19 August 2019, Revised 19 November 2019, Accepted 13 December 2019, Available online 15 December 2019. We thank Di Wu and Thomas Deffieux for helpful discussions. DM is supported by a Human Frontiers Science Program Cross-Disciplinary Postdoctoral Fellowship (Award No. LT000637/2016). This research was funded by the National Institutes of Health (grant U01NS099724 to MGS). Related research in the Shapiro laboratory is also supported by the Heritage Medical Research Institute, Burroughs Wellcome Career Award at the Scientific Interface, the Pew Scholarship in the Biomedical Sciences and the Packard Fellowship for Science and Engineering. Author contributions: David Maresca: Conceptualization, Methodology, Software, Validation, Formal Analysis, Investigation, Resources, Data Curation, Visualization, Project Administration, Writing – Original Draft, Funding Acquisition. Thomas Payen: Methodology, Software, Formal Analysis, Investigation, Resources, Data Curation, Writing – Original Draft. Audrey Lee-Gosselin: Methodology, Investigation, Project Administration. Bill Ling: Methodology, Investigation. Dina Malounda: Resources. Charlie Demené: Conceptualization, Methodology, Software, Investigation, Writing – Review and Editing. Mickaël Tanter: Conceptualization, Funding Acquisition, Writing – Review and Editing, Supervision. Mikhail G. Shapiro: Conceptualization, Formal Analysis, Funding Acquisition, Writing – Review and Editing, Supervision. Declaration of competing interest: MT is a co-founder and shareholder of Iconeus, a company commercializing ultrasound neuroimaging scanners. Other authors declare no competing financial interests.
Group:Heritage Medical Research Institute
Funders:
Funding AgencyGrant Number
Human Frontier Science ProgramLT000637/2016
NIHU01NS099724
Heritage Medical Research InstituteUNSPECIFIED
Burroughs Wellcome FundUNSPECIFIED
Pew Charitable TrustUNSPECIFIED
David and Lucile Packard FoundationUNSPECIFIED
PubMed Central ID:PMC6955150
Record Number:CaltechAUTHORS:20190815-094537072
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190815-094537072
Official Citation:David Maresca, Thomas Payen, Audrey Lee-Gosselin, Bill Ling, Dina Malounda, Charlie Demené, Mickaël Tanter, Mikhail G. Shapiro, Acoustic biomolecules enhance hemodynamic functional ultrasound imaging of neural activity, NeuroImage, Volume 209, 2020, 116467, ISSN 1053-8119, https://doi.org/10.1016/j.neuroimage.2019.116467. (http://www.sciencedirect.com/science/article/pii/S1053811919310584)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:97914
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:15 Aug 2019 16:52
Last Modified:17 Mar 2020 16:23

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