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Neurons that Function within an Integrator to Promote a Persistent Behavioral State in Drosophila

Jung, Yonil and Kennedy, Ann and Chiu, Hui and Mohammad, Farhan and Claridge-Chang, Adam and Anderson, David J. (2020) Neurons that Function within an Integrator to Promote a Persistent Behavioral State in Drosophila. Neuron, 105 (2). pp. 322-333. ISSN 0896-6273. https://resolver.caltech.edu/CaltechAUTHORS:20190815-105300612

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Abstract

Innate behaviors involve both reflexive motor programs and enduring internal states, but how these responses are coordinated by the brain is not clear. In Drosophila, male-specific P1 interneurons promote courtship song, as well as a persistent internal state that prolongs courtship and enhances aggressiveness. However, P1 neurons themselves are not persistently active. Here, we identify pCd neurons as persistently active, indirect P1 targets that are required for P1-evoked persistent courtship and aggression. Acute activation of pCd neurons alone is inefficacious but enhances and prolongs courtship or aggression promoted by female cues. Brief female exposure induces a persistent increase in male aggressiveness, an effect abrogated by interruption of pCd activity. pCd activity is not sufficient but necessary for persistent physiological activity, implying an essential role in a persistence network. Thus, P1 neurons coordinate both command-like control of courtship song and a persistent internal state of social arousal mediated by pCd neurons.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.neuron.2019.10.028DOIArticle
https://doi.org/10.1101/735985DOIDiscussion Paper
ORCID:
AuthorORCID
Kennedy, Ann0000-0002-3782-0518
Claridge-Chang, Adam0000-0002-4583-3650
Anderson, David J.0000-0001-6175-3872
Additional Information:© 2019 Elsevier Inc. Received 12 September 2019, Revised 8 October 2019, Accepted 18 October 2019, Available online 3 December 2019. We thank H. Inagaki for comments on the manuscript, B. Pfeiffer and G.M. Rubin for fly strains, A.M. Wong for help with initial development of all-optical stimulation and imaging, A. Sanchez for maintaining fly stocks, C. Chiu and X. Da for lab management, and G. Mancuso for administrative assistance. This work was supported in part by NIH grant R01 DA031389. D.J.A. is an Investigator of the Howard Hughes Medical Institute. Data and Code Availability: Source data and analysis code supporting the current study have not been deposited in a public repository, but are available from the corresponding author on request. Author Contributions: D.J.A. and Y.J. conceived the project, designed experiments, and co-wrote the manuscript; Y.J. performed all experiments, collected and analyzed data, and prepared figures; A.K. performed mathematical modeling studies; H.C. generated R41A01-AD, R41A01-DBD, and NLS-GCaMP6s flies; and F.M. and A.C.-C. provided unpublished LexAop-GtACR flies. The authors declare no competing interests.
Group:Tianqiao and Chrissy Chen Institute for Neuroscience
Funders:
Funding AgencyGrant Number
NIHR01 DA031389
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20190815-105300612
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190815-105300612
Official Citation:Yonil Jung, Ann Kennedy, Hui Chiu, Farhan Mohammad, Adam Claridge-Chang, David J. Anderson, Neurons that Function within an Integrator to Promote a Persistent Behavioral State in Drosophila, Neuron, Volume 105, Issue 2, 2020, Pages 322-333.e5, ISSN 0896-6273, https://doi.org/10.1016/j.neuron.2019.10.028. (http://www.sciencedirect.com/science/article/pii/S0896627319309237)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:97918
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:15 Aug 2019 18:17
Last Modified:28 Jan 2020 17:47

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