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Cyp1B1 expression patterns in the developing chick inner ear

Cardeña‐Núñez, Sheila and Sánchez‐Guardado, Luis Óscar and Hidalgo‐Sánchez, Matías (2020) Cyp1B1 expression patterns in the developing chick inner ear. Developmental Dynamics, 249 (3). pp. 410-424. ISSN 1058-8388. https://resolver.caltech.edu/CaltechAUTHORS:20190819-095758647

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Abstract

Background: Retinoic acid (RA) plays an important role in organogenesis as a paracrine signal through transcriptional regulation of an increasing number of known downstream target genes, regulating cell proliferation and differentiation. During the development of the inner ear, RA directly governs the morphogenesis and specification processes mainly by means of RA‐synthesizing retinaldehyde dehydrogenase (RALDH) enzymes. Interestingly, CYP1B1, a cytochrome P450 enzyme, is able to mediate the oxidative metabolisms also leading to RA generation, its expression patterns being associated with many known sites of RA activity. Results: This study describes for the first time the presence of CYP1B1 in the developing chick inner ear as a RALDH‐independent RA‐signaling mechanism. In our in situ hybridization analysis, Cyp1B1 expression was first observed in a domain located in the ventromedial wall of the otic anlagen, being included within the rostralmost aspect of an Fgf10‐positive pan‐sensory domain. As development proceeds, all identified Fgf10‐positive areas were Cyp1B1 stained, with all sensory patches being Cyp1B1 positive at stage HH34, except the macula neglecta. Conclusions: Cyp1B1 expression suggested a possible contribution of CYP1B1 action in the specification of the lateral‐to‐medial and dorsal‐to‐ventral axes of the developing chick inner ear.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/dvdy.99DOIArticle
ORCID:
AuthorORCID
Hidalgo‐Sánchez, Matías0000-0001-8573-8829
Additional Information:© 2019 Wiley Periodicals, Inc. Accepted manuscript online: 09 August 2019; Manuscript accepted: 26 July 2019; Manuscript revised: 26 July 2019; Manuscript received: 24 June 2019. Issue Online: 02 March 2020. Special Issue: 50 Years of Positional Information in Development, Disease & Regeneration. Funding Information: Junta de Extremadura. Grant Numbers: GR10152, GR15158, GR18114. Junta de Extremadura, Fondo Europeo de Desarrollo Regional, “Una manera de hacer Europa”. Grant Number: IB18046. Junta‐de‐Extremadura_Predoctoral studentship. Grant Number: PRE/08031. Spanish Ministry of Science and Innovation. Grant Number: BFU2010‐19461.
Funders:
Funding AgencyGrant Number
Junta de ExtremaduraGR10152
Junta de ExtremaduraGR15158
Junta de ExtremaduraGR18114
Junta de ExtremaduraIB18046
Fondo Europeo de Desarrollo Regional (FEDER)UNSPECIFIED
Junta de ExtremaduraPRE/08031
Ministerio de Ciencia e Innovación (MCINN)BFU2010‐19461
Subject Keywords:Cytochrome P450; Fgf10; otic patterning; otic specification; RALDH; retinoic acid; sensory patch
Issue or Number:3
Record Number:CaltechAUTHORS:20190819-095758647
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190819-095758647
Official Citation:Cardeña‐Núñez, S, Sánchez‐Guardado, LÓ, Hidalgo‐Sánchez, M. Cyp1B1 expression patterns in the developing chick inner ear. Developmental Dynamics. 2020; 249: 410–424. https://doi.org/10.1002/dvdy.99
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:97992
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:19 Aug 2019 17:06
Last Modified:05 Mar 2020 20:01

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