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Interplay of motility and polymer-driven depletion forces in the initial stages of bacterial aggregation

Porter, Michael K. and Steinberg, Asher Preska and Ismagilov, Rustem F. (2019) Interplay of motility and polymer-driven depletion forces in the initial stages of bacterial aggregation. Soft Matter, 15 (35). pp. 7071-7079. ISSN 1744-683X. https://resolver.caltech.edu/CaltechAUTHORS:20190823-085722494

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Abstract

Motile bacteria are often found in complex, polymer-rich environments in which microbes can aggregate via polymer-induced depletion forces. Bacterial aggregation has many biological implications; it can promote biofilm formation, upregulate virulence factors, and lead to quorum sensing. The steady state aggregation behavior of motile bacteria in polymer solutions has been well studied and shows that stronger depletion forces are required to aggregate motile bacteria as compared with their nonmotile analogs. However, no one has studied whether these same trends hold at the initial stages of aggregation. We use experiments and numerical calculations to investigate the polymer-induced depletion aggregation of motile Escherichia coli in polyethylene glycol solutions on short experimental timescales (∼10 min). Our work reveals that in the semi-dilute polymer concentration regime and at short timescales, in contrast to what is found at steady state, bacterial motility actually enhances aggregate formation by increasing the collision rate in viscous environments. These unexpected findings have implications for developing models of active matter, and for understanding bacterial aggregation in dynamic, biological environments, where the system may never reach steady state.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1039/c9sm00791aDOIArticle
http://www.rsc.org/suppdata/c9/sm/c9sm00791a/c9sm00791a1.pdfPublisherSupplementary Information
ORCID:
AuthorORCID
Porter, Michael K.0000-0002-0777-7563
Steinberg, Asher Preska0000-0002-8694-7224
Ismagilov, Rustem F.0000-0002-3680-4399
Additional Information:© 2019 The Royal Society of Chemistry. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. The article was received on 17 Apr 2019, accepted on 29 Jul 2019 and first published on 23 Aug 2019. Conflicts of interest: The technology described in this publication is the subject of a patent application filed by Caltech. This work was funded in part by the Army Research Office (ARO) Multidisciplinary University Research Initiative (MURI) contract #W911NF-17-1-0402, the Jacobs Institute for Molecular Engineering for Medicine, an NSF Graduate Research Fellowship DGE-144469 (to APS), and a Center for Environmental Microbial Interactions (CEMI) Caldwell Graduate Fellowship (to APS). We thank Andres Collazo and the Beckman Imaging Facility for help with imaging, and we thank Natasha Shelby for contributions to writing and editing this manuscript.
Group:Caltech Center for Environmental Microbial Interactions (CEMI), Jacobs Institute for Molecular Engineering for Medicine
Funders:
Funding AgencyGrant Number
Army Research Office (ARO)W911NF-17-1-0402
Jacobs Institute for Molecular Engineering for MedicineUNSPECIFIED
NSF Graduate Research FellowshipDGE-144469
Caltech Center for Environmental Microbial Interactions (CEMI)UNSPECIFIED
Issue or Number:35
Record Number:CaltechAUTHORS:20190823-085722494
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190823-085722494
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:98146
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Aug 2019 16:10
Last Modified:03 Oct 2019 21:38

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