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Calorimetric determination of microbial activity in low-​energy environments

Amend, Jan and Feyhl-Buska, Jayme and Wu, Fabai and Robador, Alberto and Orphan, Victoria and Finkelstein, Steven (2019) Calorimetric determination of microbial activity in low-​energy environments. In: 257th ACS National Meeting & Exposition, 31 March-4 April 2019, Orlando, FL.

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Calorimetric measurements of heat flow and total heat prodn. can inform on the energetics of microbial activity. Nanocalorimetry permits such investigations in environments (natural or lab.) where energy supplies are extremely limited and/or very few cells are metabolically active. We will present nanocalorimetry data obtained during incubations of unamended formation fluids from the oceanic crust (∼300 m deep at the Juan de Fuca Ridge flank) and the continental subsurface (∼1500 m deep at the Sanford Underground Research Facility in South Dakota). Enthalpic responses by the resident microbial communities to addn. of org. carbon, inorg. electron donors, and nutrients will also be discussed. We further used nanocalorimetry on lab. expts. with model organisms to det. the energetics assocd. with carbon, electron donor, and nutrient limitations. For example, with a sulfate reducing bacterium, we quantified the significant cost during the metabolic transition from ammonium assimilation to N_2 fixation. In another set of expts., with an evolving population of E. coli mutants that express the GASP (growth advantage in stationary phase) phenotype, we recorded distinct heat events assocd. with the emergence of nascent mutant populations.

Item Type:Conference or Workshop Item (Paper)
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URLURL TypeDescription Website
Amend, Jan0000-0003-4953-7776
Wu, Fabai0000-0001-5812-5621
Orphan, Victoria0000-0002-5374-6178
Finkelstein, Steven0000-0001-8519-1130
Additional Information:© 2019 American Chemical Society.
Record Number:CaltechAUTHORS:20190830-092706647
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:98365
Deposited By: Tony Diaz
Deposited On:30 Aug 2019 16:30
Last Modified:09 Mar 2020 13:18

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