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Development of a bio‐MEMS device for electrical and mechanical conditioning and characterization of cell sheets for myocardial repair

Roberts, Erin G. and Kleptsyn, Vladimir F. and Roberts, Gregory D. and Mossburg, Katherine J. and Feng, Bei and Domian, Ibrahim J. and Emani, Sitaram M. and Wong, Joyce Y. (2019) Development of a bio‐MEMS device for electrical and mechanical conditioning and characterization of cell sheets for myocardial repair. Biotechnology and Bioengineering, 116 (11). pp. 3098-3111. ISSN 0006-3592. https://resolver.caltech.edu/CaltechAUTHORS:20190830-104440699

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Abstract

Here we propose a bio‐MEMS device designed to evaluate contractile force and conduction velocity of cell sheets in response to mechanical and electrical stimulation of the cell source as it grows to form a cellular sheet. Moreover, the design allows for the incorporation of patient‐specific data and cell sources. An optimized device would allow cell sheets to be cultured, characterized, and conditioned to be compatible with a specific patient's cardiac environment in vitro, before implantation. This design draws upon existing methods in the literature but makes an important advance by combining the mechanical and electrical stimulation into a single system for optimized cell sheet growth. The device has been designed to achieve cellular alignment, electrical stimulation, mechanical stimulation, conduction velocity readout, contraction force readout, and eventually cell sheet release. The platform is a set of comb electrical contacts consisting of three‐dimensional walls made of polydimethylsiloxane and coated with electrically conductive metals on the tops of the walls. Not only do the walls serve as a method for stimulating cells that are attached to the top, but their geometry is tailored such that they are flexible enough to be bent by the cells and used to measure force. The platform can be stretched via a linear actuator setup, allowing for simultaneous electrical and mechanical stimulation that can be derived from patient‐specific clinical data.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/bit.27123DOIArticle
ORCID:
AuthorORCID
Roberts, Erin G.0000-0001-8985-8799
Wong, Joyce Y.0000-0002-3526-6381
Additional Information:© 2019 Wiley Periodicals, Inc. Version of Record online: 06 August 2019; Accepted manuscript online: 18 July 2019; Manuscript accepted: 09 July 2019; Manuscript revised: 09 May 2019; Manuscript received: 26 January 2019.
Subject Keywords:beat contractility; bio‐MEMS; conduction velocity; electromechanical stimulation; myocardium
Issue or Number:11
Record Number:CaltechAUTHORS:20190830-104440699
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190830-104440699
Official Citation:Roberts, EG, Kleptsyn, VF, Roberts, GD, et al. Development of a bio‐MEMS Device for Electrical and Mechanical Conditioning and Characterization of Cell Sheets for Myocardial Repair. Biotechnology and Bioengineering. 2019; 116: 3098–3111. https://doi.org/10.1002/bit.27123
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:98370
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 Aug 2019 18:11
Last Modified:07 Oct 2019 16:09

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