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The Developmental Transcriptome of Aedes albopictus, a Major Worldwide Human Disease Vector

Gamez, Stephanie and Antoshechkin, Igor and Mendez-Sanchez, Stelia C. and Akbari, Omar S. (2020) The Developmental Transcriptome of Aedes albopictus, a Major Worldwide Human Disease Vector. G3, 10 (3). pp. 1051-1062. ISSN 2160-1836. PMCID PMC7056973. https://resolver.caltech.edu/CaltechAUTHORS:20190904-144412708

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Abstract

Aedes albopictus mosquitoes are important vectors for a number of human pathogens including the Zika, dengue, and chikungunya viruses. Capable of displacing Aedes aegypti populations, this mosquito adapts to cooler environments which increases its geographical range and transmission potential. There are limited control strategies for Aedes albopictus mosquitoes which is likely attributed to the lack of comprehensive biological studies on this emerging vector. To fill this void, here using RNAseq we characterized Aedes albopictus mRNA expression profiles at 34 distinct time points throughout development providing the first high-resolution comprehensive view of the developmental transcriptome of this worldwide human disease vector. This enabled us to identify several patterns of shared gene expression among tissues as well as sex-specific expression patterns. To illuminate the similarities and differences with Aedes aegypti, a related human disease vector, we also performed a comparative analysis between the two developmental transcriptomes, identifying life stages where the two species exhibit similar and distinct gene expression patterns. These findings provide insights into the similarities and differences between Aedes albopictus and Aedes aegypti mosquito biology. In summary, the results generated from this study should form the basis for future investigations on the biology of Aedes albopictus and provide a gold mine resource for the development of transgene-based vector control strategies.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1534/g3.119.401006DOIArticle
https://doi.org/10.1101/753962DOIDiscussion Paper
https://doi.org/10.25387/g3.11653461DOISupplementary Material
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc7056973/PubMed CentralArticle
ORCID:
AuthorORCID
Gamez, Stephanie0000-0003-0665-0066
Antoshechkin, Igor0000-0002-9934-3040
Mendez-Sanchez, Stelia C.0000-0002-2402-3632
Akbari, Omar S.0000-0002-6853-9884
Alternate Title:Developmental Transcriptome of Ae. albopictus, a Major Worldwide Human Disease Vector
Additional Information:© 2020 Gamez et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Manuscript received December 12, 2019; accepted for publication January 14, 2020; published Early Online January 21, 2020. This work was supported by funding from generous UCSD lab startup funds and NIH grants R21AI142443 and K22AI113060 awarded to O.S.A. We thank Vijaya Kumar for help with library preparations. Transcriptome sequencing was performed at the Millard and Muriel Jacobs Genetics and Genomics Laboratory at the California Institute of Technology. We also thank Dr. J. Wakoli Wekesa and the San Gabriel Valley Mosquito and Vector Control District for providing us with the Ae. albopictus mosquito eggs used in this study. O.S.A., and I.A. conceptualized the study. S.G. performed all sample preparations. I.A, S.G., and S.C.M performed the various bioinformatic analysis in the study. All authors contributed to the writing, analyzed the data, and approved the final manuscript.
Group:Millard and Muriel Jacobs Genetics and Genomics Laboratory
Funders:
Funding AgencyGrant Number
University of California, San DiegoUNSPECIFIED
NIHR21AI142443
NIHK22AI113060
Subject Keywords:Aedes albopictus, transcriptome, development, Zika, dengue, Aedes aegypti, RNA-seq
Issue or Number:3
PubMed Central ID:PMC7056973
Record Number:CaltechAUTHORS:20190904-144412708
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190904-144412708
Official Citation:The Developmental Transcriptome of Aedes albopictus, a Major Worldwide Human Disease Vector. Stephanie Gamez, Igor Antoshechkin, Stelia C. Mendez-Sanchez and Omar S. Akbari. G3: GENES, GENOMES, GENETICS March 1, 2020 vol. 10 no. 3 1051-1062; https://doi.org/10.1534/g3.119.401006
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:98415
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Sep 2019 21:50
Last Modified:31 Mar 2020 18:34

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