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Assessment of Robustness to Temperature in a Negative Feedback Loop and a Feedforward Loop

Patel, Abhilash and Murray, Richard M. and Sen, Shaunak (2020) Assessment of Robustness to Temperature in a Negative Feedback Loop and a Feedforward Loop. ACS Synthetic Biology, 9 (7). pp. 1581-1590. ISSN 2161-5063. https://resolver.caltech.edu/CaltechAUTHORS:20190918-100800913

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Abstract

Robustness to temperature variation is an important specification in biomolecular circuit design. While the cancellation of parametric temperature dependencies has been shown to improve the temperature robustness of the period in a synthetic oscillator design, the performance of other biomolecular circuit designs in different temperature conditions is relatively unclear. Using a combination of experimental measurements and mathematical models, we assessed the temperature robustness of two biomolecular circuit motifs—a negative feedback loop and a feedforward loop. We found that the measured responses of both the circuits changed with temperature, both in the amplitude and in the transient response. We also found that, in addition to the cancellation of parametric temperature dependencies, certain parameter regimes could facilitate the temperature robustness of the negative feedback loop, although at a performance cost. We discuss these parameter regimes in the context of the measured data for the negative feedback loop. These results should help develop a framework for assessing and designing temperature robustness in biomolecular circuits.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/acssynbio.0c00023DOIArticle
https://doi.org/10.1101/774042DOIDiscussion Paper
ORCID:
AuthorORCID
Patel, Abhilash0000-0001-6319-658X
Murray, Richard M.0000-0002-5785-7481
Sen, Shaunak0000-0002-1412-8633
Additional Information:© 2020 American Chemical Society. Received: January 13, 2020; Published: June 11, 2020. We thank C. A. Hayes for her help with the cell-free experiments. We are grateful to the referees for their valuable comments. A. Patel acknowledges financial support from a DeitY Fellowship. This work was supported in part by Science and Engineering Research Board Grant No. SB/FTP/ETA-0152/2013. The authors declare no competing financial interest.
Funders:
Funding AgencyGrant Number
Indian Institute of TechnologyUNSPECIFIED
Science and Engineering Research Board (SERB)SB/FTP/ETA-0152/2013
Subject Keywords:synthetic biology, temperature robustness, negative feedback, feedforward loop
Issue or Number:7
Record Number:CaltechAUTHORS:20190918-100800913
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20190918-100800913
Official Citation:Assessment of Robustness to Temperature in a Negative Feedback Loop and a Feedforward Loop. Abhilash Patel, Richard M. Murray, and Shaunak Sen. ACS Synthetic Biology 2020 9 (7), 1581-1590; DOI: 10.1021/acssynbio.0c00023
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:98716
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:18 Sep 2019 18:02
Last Modified:17 Jul 2020 22:04

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