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Light-Dependent Translocation of Arrestin in the Absence of Rhodopsin Phosphorylation and Transducin Signaling

Mendez, Ana and Lem, Janis and Simon, Melvin and Chen, Jeannie (2003) Light-Dependent Translocation of Arrestin in the Absence of Rhodopsin Phosphorylation and Transducin Signaling. Journal of Neuroscience, 23 (8). pp. 3124-3129. ISSN 0270-6474. PMCID PMC6742335. doi:10.1523/jneurosci.23-08-03124.2003.

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Visual arrestin plays a crucial role in the termination of the light response in vertebrate photoreceptors by binding selectively to light-activated, phosphorylated rhodopsin. Arrestin localizes predominantly to the inner segments and perinuclear region of dark-adapted rod photoreceptors, whereas light induces redistribution of arrestin to the rod outer segments. The mechanism by which arrestin redistributes in response to light is not known, but it is thought to be associated with the ability of arrestin to bind photolyzed, phosphorylated rhodopsin in the outer segment. In this study, we show that light-driven translocation of arrestin is unaffected in two different mouse models in which rhodopsin phosphorylation is lacking. We further show that arrestin movement is initiated by rhodopsin but does not require transducin signaling. These results exclude passive diffusion and point toward active transport as the mechanism for light-dependent arrestin movement in rod photoreceptor cells.

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Additional Information:© 2003 Society for Neuroscience. Received Nov. 13, 2002; revised Jan. 31, 2003; accepted Feb. 4, 2003. This work was supported by National Institutes of Health (NIH) Grants EY12155, EY12703 (J.C.), EY12008, and AG12288 (M.S.), NIH Specialized Centers of Research in Ischemic Heart Disease (J.L.), the Beckman Macular Research Center (J.C.), and the Massachusetts Lions Eye Research Fund (J.L.). J.C. is a Research to Prevent Blindness James S. Adams Scholar and a Beckman Investigator. We thank Dr. N. Rao for providing the arrestin antibody and the Specialized Imaging Core of the Doheny Eye Institute (National Eye Institute Grant EY03040) for their technical support and guidance.
Funding AgencyGrant Number
Beckman Macular Research CenterUNSPECIFIED
Massachusetts Lions Eye Research FundUNSPECIFIED
Research to Prevent BlindnessUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Subject Keywords:arrestin; transducin; RPE65; rhodopsin phosphorylation; rod photoreceptor; retina
Issue or Number:8
PubMed Central ID:PMC6742335
Record Number:CaltechAUTHORS:20190926-160427114
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Official Citation:Light-Dependent Translocation of Arrestin in the Absence of Rhodopsin Phosphorylation and Transducin Signaling. Ana Mendez, Janis Lem, Melvin Simon, Jeannie Chen. Journal of Neuroscience 15 April 2003, 23 (8) 3124-3129; DOI: 10.1523/JNEUROSCI.23-08-03124.2003
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:98889
Deposited By: Tony Diaz
Deposited On:26 Sep 2019 23:15
Last Modified:02 Mar 2022 00:47

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