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A dynamic pattern of local auxin sources is required for root regeneration

Matosevich, Rotem and Cohen, Itay and Gil-Yarom, Naama and Modrego, Abelardo and Verna, Carla and Scarpella, Enrico and Efroni, Idan (2019) A dynamic pattern of local auxin sources is required for root regeneration. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20191001-104800301

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Abstract

Following removal of its stem cell niche, the root meristem can regenerate by recruitment of remnant cells from the stump. Regeneration is initiated by rapid accumulation of auxin near the injury site but the source of this auxin is unknown. Here, we show that auxin accumulation arises from the activity of multiple auxin biosynthetic sources that are newly specified near the cut site and that their continuous activity is required for the regeneration process. Auxin synthesis is highly localized and PIN-mediate transport is dispensable for auxin accumulation and tip regeneration. Roots lacking the activity of the regeneration competence factor ERF115, or that are dissected at a zone of low-regeneration potential, fail to activate local auxin sources. Remarkably, restoring auxin supply is sufficient to confer regeneration capacity to these recalcitrant tissues. We suggest that regeneration competence relies on the ability to specify new local auxin sources in a precise spatio-temporal pattern.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/783480DOIDiscussion Paper
ORCID:
AuthorORCID
Verna, Carla0000-0001-5312-5470
Scarpella, Enrico0000-0003-2962-0329
Efroni, Idan0000-0002-0219-8685
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. We thank Jose Alonso, Anna Stepanova and Lieven de Veylder for sharing research material. We thank Yuval Eshed and Sigal Savaldi-Goldstein for comments and discussions. IE is supported by the Israeli Science Foundation (ISF966/17) and the Howard Hughes Medical Institute International Research Scholar Grant (55008730). Accession Numbers: RNA-seq data were deposited in GEO (GEO series).
Funders:
Funding AgencyGrant Number
Israel Science FoundationISF966/17
Howard Hughes Medical Institute (HHMI)55008730
Record Number:CaltechAUTHORS:20191001-104800301
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20191001-104800301
Official Citation:A dynamic pattern of local auxin sources is required for root regeneration Rotem Matosevich, Itay Cohen, Naama Gil-Yarom, Abelardo Modrego, Carla Verna, Enrico Scarpella, Idan Efroni bioRxiv 783480; doi: https://doi.org/10.1101/783480
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:98982
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:01 Oct 2019 20:52
Last Modified:03 Oct 2019 21:46

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