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Bacterial flagellar motor PL-ring disassembly sub-complexes are widespread and ancient

Kaplan, Mohammed and Sweredoski, Michael J. and Rodrigues, João and Tocheva, Elitza I. and Chang, Yi-Wei and Ortega, Davi R. and Beeby, Morgan and Jensen, Grant J. (2019) Bacterial flagellar motor PL-ring disassembly sub-complexes are widespread and ancient. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20191002-094950568

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Abstract

The bacterial flagellar motor is an amazing nanomachine. Understanding how such complex structures arose is crucial to our understanding of cellular evolution. We and others recently reported that in several Gammaproteobacterial species, a relic sub-complex comprising the P- and L-rings persists in the outer membrane after flagellum disassembly. Imaging nine additional species with cryo-electron tomography, here we show that this sub-complex persists after flagellum disassembly in other phyla as well. Bioinformatic analyses fail to show evidence of any recent horizontal transfers of the P- and L-ring genes, suggesting that this sub-complex and its persistence is an ancient and conserved feature of the flagellar motor. We hypothesize that one function of the P- and L-rings is to seal the outer membrane after motor disassembly.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/786715DOIDiscussion Paper
ORCID:
AuthorORCID
Kaplan, Mohammed0000-0002-0759-0459
Sweredoski, Michael J.0000-0003-0878-3831
Rodrigues, João0000-0001-9796-3193
Tocheva, Elitza I.0000-0002-4869-8319
Chang, Yi-Wei0000-0003-2391-473X
Ortega, Davi R.0000-0002-8344-2335
Beeby, Morgan0000-0001-6413-9835
Jensen, Grant J.0000-0003-1556-4864
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. This work was supported by NIH grant R35 GM122588 to GJJ. Cryo-EM work was done in the Beckman Institute Resource Center for Transmission Electron Microscopy at Caltech. M.K. acknowledges a Rubicon postdoctoral fellowship from De Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO). Ariane Briegel kindly helped in collecting part of the data. We thank Catherine M. Oikonomou for reading the manuscript and for the insightful discussions. We thank Dr. Pat Zambryski from the University of California, Berkeley for providing us with A. longum strain used in this study.
Funders:
Funding AgencyGrant Number
NIHR35 GM122588
Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO)UNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Record Number:CaltechAUTHORS:20191002-094950568
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20191002-094950568
Official Citation:Bacterial flagellar motor PL-ring disassembly sub-complexes are widespread and ancient Mohammed Kaplan, Michael J Sweredoski, Joao Rodrigues, Elitza I Tocheva, Yi-Wei Chang, Davi R Ortega, Morgan Beeby, Grant J Jensen bioRxiv 786715; doi: https://doi.org/10.1101/786715
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:99008
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:02 Oct 2019 17:29
Last Modified:03 Oct 2019 21:46

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