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Genome Elimination Mediated by Gene Expression from a Selfish Chromosome

Dalla Benetta, Elena and Antoshechkin, Igor and Yang, Ting and Nguyen, Hoa Quang My and Ferree, Patrick M. and Akbari, Omar S. (2020) Genome Elimination Mediated by Gene Expression from a Selfish Chromosome. Science Advances, 6 (14). Art. No. eaaz9808. ISSN 2375-2548. PMCID PMC7124933. https://resolver.caltech.edu/CaltechAUTHORS:20191007-133644493

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Abstract

Numerous plants and animals harbor selfish B chromosomes that “drive” or transmit themselves at super-Mendelian frequencies, despite long-term fitness costs to the organism. Currently, it is unknown how B chromosome drive is mediated, and whether B-gene expression plays a role. We used modern sequencing technologies to analyze the fine-scale sequence composition and expression of paternal sex ratio (PSR), a B chromosome in the jewel wasp Nasonia vitripennis. PSR causes female-to-male conversion by destroying the sperm’s hereditary material in young embryos to drive. Using RNA interference, we demonstrate that testis-specific expression of a PSR-linked gene, named haploidizer, facilitates this genome elimination–and–sex conversion effect. haploidizer encodes a putative protein with a DNA binding domain, suggesting a functional link with the sperm-derived chromatin.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1126/sciadv.aaz9808DOIArticle
https://advances.sciencemag.org/cgi/content/full/6/14/eaaz9808/DC1PublisherSupplementary Materials
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7124933PubMed CentralArticle
https://doi.org/10.1101/793273DOIDiscussion Paper
ORCID:
AuthorORCID
Dalla Benetta, Elena0000-0003-2556-8500
Antoshechkin, Igor0000-0002-9934-3040
Yang, Ting0000-0003-2301-2525
Ferree, Patrick M.0000-0002-4315-4363
Akbari, Omar S.0000-0002-6853-9884
Additional Information:© 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). Submitted 24 October 2019; Accepted 14 January 2020; Published 3 April 2020. We thank V. Kumar for help with genomic library preparations. Sequencing was performed at the Millard and Muriel Jacobs Genetics and Genomics Laboratory at the California Institute of Technology. This work was supported in part by funding from generous UCSD laboratory startup funds awarded to O.S.A. and NSF funding awarded to P.M.F. (NSF-1451839). Author contributions: O.S.A. and P.M.F. conceptualized the study. E.D.B., I.A., T.Y., P.M.F., and H.Q.M.N. performed the various genetic, cytological, and molecular experiments and the bioinformatic analyses in the study. All authors contributed to the writing, analyzed the data, and approved the final manuscript. Competing interests: The authors declare that they have no significant competing financial, professional, or personal interests that might have influenced the performance or presentation of the work described. Data and materials availability: All Illumina, PacBio, and Oxford Nanopore sequencing data can be found at NCBI under accession number PRJNA575073 (https://dataview.ncbi.nlm.nih.gov/object/PRJNA575073?reviewer=uslhbl0iop93gab77lb31ts0n). Previously published RNA-seq data (18) were also used in this study. They can be found under the accession number PRJNA212516 (www.ncbi.nlm.nih.gov/bioproject/PRJNA212516). All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. Additional data related to this paper may be requested from the authors.
Group:Millard and Muriel Jacobs Genetics and Genomics Laboratory
Funders:
Funding AgencyGrant Number
University of California, San DiegoUNSPECIFIED
NSFMCB-1451839
Issue or Number:14
PubMed Central ID:PMC7124933
Record Number:CaltechAUTHORS:20191007-133644493
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20191007-133644493
Official Citation:Genome Elimination Mediated by Gene Expression from a Selfish Chromosome. Elena Dalla Benetta, Igor Antoshechkin, Ting Yang, Hoa Quang My Nguyen, Patrick M. Ferree, Omar S. Akbari. bioRxiv 793273; doi: https://doi.org/10.1101/793273
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:99117
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Oct 2019 20:47
Last Modified:15 Apr 2020 17:53

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