Genome Elimination Mediated by Gene Expression from a Selfish Chromosome

Dalla Benetta, Elena and Antoshechkin, Igor and Yang, Ting and Nguyen, Hoa Quang My and Ferree, Patrick M. and Akbari, Omar S. (2020) Genome Elimination Mediated by Gene Expression from a Selfish Chromosome. Science Advances, 6 (14). Art. No. eaaz9808. ISSN 2375-2548. PMCID PMC7124933. https://resolver.caltech.edu/CaltechAUTHORS:20191007-133644493

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Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20191007-133644493

Abstract

Numerous plants and animals harbor selfish B chromosomes that “drive” or transmit themselves at super-Mendelian frequencies, despite long-term fitness costs to the organism. Currently, it is unknown how B chromosome drive is mediated, and whether B-gene expression plays a role. We used modern sequencing technologies to analyze the fine-scale sequence composition and expression of paternal sex ratio (PSR), a B chromosome in the jewel wasp Nasonia vitripennis. PSR causes female-to-male conversion by destroying the sperm’s hereditary material in young embryos to drive. Using RNA interference, we demonstrate that testis-specific expression of a PSR-linked gene, named haploidizer, facilitates this genome elimination–and–sex conversion effect. haploidizer encodes a putative protein with a DNA binding domain, suggesting a functional link with the sperm-derived chromatin.

Item Type:

Article

Related URLs:

URL	URL Type	Description
https://doi.org/10.1126/sciadv.aaz9808	DOI	Article
https://advances.sciencemag.org/cgi/content/full/6/14/eaaz9808/DC1	Publisher	Supplementary Materials
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7124933	PubMed Central	Article
https://doi.org/10.1101/793273	DOI	Discussion Paper

ORCID:

Author	ORCID
Dalla Benetta, Elena	0000-0003-2556-8500
Antoshechkin, Igor	0000-0002-9934-3040
Yang, Ting	0000-0003-2301-2525
Ferree, Patrick M.	0000-0002-4315-4363
Akbari, Omar S.	0000-0002-6853-9884

Additional Information:

© 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). Submitted 24 October 2019; Accepted 14 January 2020; Published 3 April 2020. We thank V. Kumar for help with genomic library preparations. Sequencing was performed at the Millard and Muriel Jacobs Genetics and Genomics Laboratory at the California Institute of Technology. This work was supported in part by funding from generous UCSD laboratory startup funds awarded to O.S.A. and NSF funding awarded to P.M.F. (NSF-1451839). Author contributions: O.S.A. and P.M.F. conceptualized the study. E.D.B., I.A., T.Y., P.M.F., and H.Q.M.N. performed the various genetic, cytological, and molecular experiments and the bioinformatic analyses in the study. All authors contributed to the writing, analyzed the data, and approved the final manuscript. Competing interests: The authors declare that they have no significant competing financial, professional, or personal interests that might have influenced the performance or presentation of the work described. Data and materials availability: All Illumina, PacBio, and Oxford Nanopore sequencing data can be found at NCBI under accession number PRJNA575073 (https://dataview.ncbi.nlm.nih.gov/object/PRJNA575073?reviewer=uslhbl0iop93gab77lb31ts0n). Previously published RNA-seq data (18) were also used in this study. They can be found under the accession number PRJNA212516 (www.ncbi.nlm.nih.gov/bioproject/PRJNA212516). All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. Additional data related to this paper may be requested from the authors.

Group:

Millard and Muriel Jacobs Genetics and Genomics Laboratory

Funders:

Funding Agency	Grant Number
University of California, San Diego	UNSPECIFIED
NSF	MCB-1451839

Issue or Number:

PubMed Central ID:

PMC7124933

Record Number:

CaltechAUTHORS:20191007-133644493

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:20191007-133644493

Official Citation:

Genome Elimination Mediated by Gene Expression from a Selfish Chromosome. Elena Dalla Benetta, Igor Antoshechkin, Ting Yang, Hoa Quang My Nguyen, Patrick M. Ferree, Omar S. Akbari. bioRxiv 793273; doi: https://doi.org/10.1101/793273

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No commercial reproduction, distribution, display or performance rights in this work are provided.

ID Code:

99117

Collection:

CaltechAUTHORS

Deposited By:

Tony Diaz

Deposited On:

07 Oct 2019 20:47

Last Modified:

15 Apr 2020 17:53

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