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A rhlI 5′ UTR-Derived sRNA Regulates RhlR-Dependent Quorum Sensing in Pseudomonas aeruginosa

Thomason, Maureen K. and Voichek, Maya and Dar, Daniel and Addis, Victoria and Fitzgerald, David and Gottesman, Susan and Sorek, Rotem and Greenberg, E. Peter (2019) A rhlI 5′ UTR-Derived sRNA Regulates RhlR-Dependent Quorum Sensing in Pseudomonas aeruginosa. mBio, 10 (5). Art. No. e02253-19. ISSN 2150-7511. PMCID PMC6786874. https://resolver.caltech.edu/CaltechAUTHORS:20191008-075652504

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Abstract

N-Acyl homoserine lactone (AHL) quorum sensing (QS) controls expression of over 200 genes in Pseudomonas aeruginosa. There are two AHL regulatory systems: the LasR-LasI circuit and the RhlR-RhlI system. We mapped transcription termination sites affected by AHL QS in P. aeruginosa, and in doing so we identified AHL-regulated small RNAs (sRNAs). Of interest, we noted that one particular sRNA was located within the rhlI locus. We found that rhlI, which encodes the enzyme that produces the AHL N-butanoyl-homoserine lactone (C4-HSL), is controlled by a 5′ untranslated region (UTR)-derived sRNA we name RhlS. We also identified an antisense RNA encoded opposite the beginning of the rhlI open reading frame, which we name asRhlS. RhlS accumulates as wild-type cells enter stationary phase and is required for the production of normal levels of C4-HSL through activation of rhlI translation. RhlS also directly posttranscriptionally regulates at least one other unlinked gene, fpvA. The asRhlS appears to be expressed at maximal levels during logarithmic growth, and we suggest RhlS may act antagonistically to the asRhlS to regulate rhlI translation. The rhlI-encoded sRNAs represent a novel aspect of RNA-mediated tuning of P. aeruginosa QS.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1128/mBio.02253-19DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6786874/PubMed CentralArticle
ORCID:
AuthorORCID
Voichek, Maya0000-0002-0508-0682
Dar, Daniel0000-0002-6650-5488
Fitzgerald, David0000-0002-6589-0126
Sorek, Rotem0000-0002-3872-4982
Greenberg, E. Peter0000-0001-9474-8041
Additional Information:© 2019 Thomason et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Received August 31, 2019. Accepted September 11, 2019. Published online October 8, 2019. We thank M. Schuster, A. Dandakar, P. Mandin, E. Fozo, and H. Schweizer for providing strains and plasmids. This work was supported by U.S. Public Health Service (USPHS) grant GM59026 to E.P.G. and European Research Council (ERC) grant ERC-CoG 681203 to R.S. Support for M.K.T. was provided by a Cystic Fibrosis Foundation Postdoctoral Fellowship. M.V. is a Clore Scholar and was supported by the Clore Israel Foundation.
Funders:
Funding AgencyGrant Number
NIHGM59026
European Research Council (ERC)681203
Cystic Fibrosis FoundationUNSPECIFIED
Clore Israel FoundationUNSPECIFIED
Issue or Number:5
PubMed Central ID:PMC6786874
Record Number:CaltechAUTHORS:20191008-075652504
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20191008-075652504
Official Citation:A rhlI 5′ UTR-Derived sRNA Regulates RhlR-Dependent Quorum Sensing in Pseudomonas aeruginosa Maureen K. Thomason, Maya Voichek, Daniel Dar, Victoria Addis, David Fitzgerald, Susan Gottesman, Rotem Sorek, E. Peter Greenberg mBio Oct 2019, 10 (5) e02253-19; DOI: 10.1128/mBio.02253-19
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:99134
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Oct 2019 16:32
Last Modified:05 Nov 2019 18:17

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