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Amino acid sequences of the epsilon and alpha E globins of HbE, a minor early embryonic hemoglobin of the chicken

Chapman, Barbara S. and Hood, Leroy E. and Tobin, Allan J. (1982) Amino acid sequences of the epsilon and alpha E globins of HbE, a minor early embryonic hemoglobin of the chicken. Journal of Biological Chemistry, 257 (2). pp. 643-650. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:CHAjbc82a

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Abstract

We have determined amino acid sequences for the alpha-like and beta- like globin components of HbE, one of the two minor hemoglobins in early chick embryos. The complete primary structure of the epsilon chain differs at 18 positions from the adult chicken beta globin, but there are no changes in heme-binding residues, alpha 1 beta 2 contact positions, or allosteric regulatory sites. By amino acid sequence analysis, we have identified a new alpha-like globin that we have called alpha E. The alpha E globin chain differs from the major adult alpha A chain at 22 amino acid positions. This paper discusses the structural and implied functional characteristics of these globins and presents hypotheses regarding the possible role of minor embryonic hemoglobins.


Item Type:Article
Additional Information:Copyright © 1982 by the American Society for Biochemistry and Molecular Biology. (Received for publication, April 24, 1981, and in revised form, September 9, 1981) We sincerely thank Dr. James Schilling for teaching one of us (B.S.C.) amino acid sequencing techniques, Margaret Kowalczyk for illustration, and Lori Erdley for patient assistance in the preparation of this manuscript. The critical comments of Dr. Allan C. Wilson and Sandra Martin were invaluable. We especially appreciate the many stimulating discussions with Dr. J.D. Engel and thank him for sharing unpublished manuscripts with us. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. [B.S.C. was the] Recipient of National Research Service Award HL05553. [L.E.H. was the] Recipient of National Institutes of Health Grant GM-06965. [A.J.T. was the] Recipient of National Science Foundation Grants PCM 76-02859 and 78-20767. Portions of this paper (including “Experimental Procedures,” “Results,” Figs. 1-9, and Table I) are presented in miniprint at the end of this paper.
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Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:CHAjbc82a
Alternative URL:http://www.jbc.org/cgi/content/abstract/257/2/643
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ID Code:9929
Collection:CaltechAUTHORS
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Deposited On:27 Mar 2008
Last Modified:26 Dec 2012 09:54

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