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Sympathetic Effects of Internal Carotid Nerve Manipulation on Choroidal Vascularity and Related Measures

Martinez-Camarillo, Juan-Carlos and Spee, Christine K. and Chen, Michael and Rodriguez, Anthony and Nimmagadda, Kiran and Trujillo-Sanchez, Gloria Paulina and Hinton, David R. and Giarola, Alessandra and Pikov, Victor and Sridhar, Arun and Humayun, Mark S. and Weitz, Andrew C. (2019) Sympathetic Effects of Internal Carotid Nerve Manipulation on Choroidal Vascularity and Related Measures. Investigative Ophthalmology and Visual Science, 60 (13). pp. 4303-4309. ISSN 1552-5783. https://resolver.caltech.edu/CaltechAUTHORS:20191022-104814157

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Abstract

Purpose: To investigate specific effects of denervation and stimulation of the internal carotid nerve (ICN) on the choroid and retina. Methods: Female Sprague Dawley rats underwent unilateral ICN transection (n = 20) or acute ICN electrical stimulation (n = 7). Rats in the denervation group were euthanized 6 weeks after nerve transection, and eyes were analyzed for changes in choroidal vascularity (via histomorphometry) or angiogenic growth factors and inflammatory markers (via ELISA). Rats in the stimulation group received acute ICN electrical stimulation with a bipolar cuff electrode over a range of stimulus amplitudes, frequencies, and pulse widths. Choroidal blood flow and pupil diameter were monitored before, during, and after stimulation. Results: Six weeks after unilateral ICN transection, sympathectomized choroids exhibited increased vascularity, defined as the percentage of choroidal surface area occupied by blood vessel lumina. Vascular endothelial growth factor (VEGF) and VEGF receptor-2 (VEGFR-2) protein levels in denervated choroids were 61% and 124% higher than in contralateral choroids, respectively. TNF-α levels in denervated retinas increased by 3.3-fold relative to levels in contralateral retinas. In animals undergoing acute ICN electrical stimulation, mydriasis and reduced choroidal blood flow were observed in the ipsilateral eye. The magnitude of the reduction in blood flow correlated positively with stimulus frequency. Conclusions: Modulation of ICN activity reveals a potential role of the ocular sympathetic system in regulating endpoints related to neovascular diseases of the eye.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1167/iovs.18-25613DOIArticle
ORCID:
AuthorORCID
Humayun, Mark S.0000-0002-5830-5208
Additional Information:© 2019 The Authors. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. The authors thank Danhong Zhu for her help with the ELISA assays. Supported by a grant from Galvani Bioelectronics to ACW, MSH, and DRH, as well as unrestricted departmental support to the USC Roski Eye Institute from Research to Prevent Blindness. Disclosure: J.-C. Martinez-Camarillo, None; C.K. Spee, None; M. Chen, None; A. Rodriguez, None; K. Nimmagadda, None; G.P. Trujillo-Sanchez, None; D.R. Hinton, Galvani Bioelectronics (F); A. Giarola, P; V. Pikov, P; A. Sridhar, E, P; M.S. Humayun, Galvani Bioelectronics (F), P; A.C. Weitz, Galvani Bioelectronics (F), P
Funders:
Funding AgencyGrant Number
Galvani BioelectronicsUNSPECIFIED
University of Southern CaliforniaUNSPECIFIED
Subject Keywords:internal carotid nerve, choroidal vascularity, sympathectomy, electrical stimulation
Issue or Number:13
Record Number:CaltechAUTHORS:20191022-104814157
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20191022-104814157
Official Citation:Juan-Carlos Martinez-Camarillo, Christine K. Spee, Michael Chen, Anthony Rodriguez, Kiran Nimmagadda, Gloria Paulina Trujillo-Sanchez, David R. Hinton, Alessandra Giarola, Victor Pikov, Arun Sridhar, Mark S. Humayun, Andrew C. Weitz; Sympathetic Effects of Internal Carotid Nerve Manipulation on Choroidal Vascularity and Related Measures. Invest. Ophthalmol. Vis. Sci. 2019; 60(13): 4303-4309. doi: 10.1167/iovs.18-25613
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:99387
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:22 Oct 2019 20:19
Last Modified:09 Mar 2020 13:18

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