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A three-component theory of sedimentation equilibrium in a density gradient

Hearst, John E. and Vinograd, Jerome (1961) A three-component theory of sedimentation equilibrium in a density gradient. Proceedings of the National Academy of Sciences of the United States of America, 47 (7). pp. 999-1004. ISSN 0027-8424. doi:10.1073/pnas.47.7.999.

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In the original analysis (1) of the behavior of macromolecules and viruses in a density gradient at equilibrium in the ultracentrifuge, only two incompressible components were considered to be present. These were the polymer and the solvent which forms the density gradient. The pressure-dependent terms and the cross terms in the chemical potential which describe the chemical reactions between the polymer and the solute were neglected. It has been pointed out from theoretical considerations (2-4) that these terms are important. In experimental studies of the buoyant behavior of bovine mercaptalbumin in aqueous cesium chloride,(5) it was observed that both solvation and pressure effects are large.

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Additional Information:© 1961 by the National Academy of Sciences. Communicated by Linus Pauling, May 29, 1961. This investigation was supported in part by research grant H-3394 from the National Institutes of Health, U.S. Public Health Service. [J.E.H. was a] Predoctoral Fellow of the National Science Foundation. Gates and Crellin Laboratories of Chemistry, Contribution No. 2705.
Issue or Number:7
Record Number:CaltechAUTHORS:HEApnas61b
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:9947
Deposited By: Tony Diaz
Deposited On:28 Mar 2008
Last Modified:08 Nov 2021 21:03

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