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Synaptic Control of Spiking in Cerebellar Purkinje Cells: Dynamic Current Clamp Based on Model Conductances

Jaeger, Dieter and Bower, James M. (1999) Synaptic Control of Spiking in Cerebellar Purkinje Cells: Dynamic Current Clamp Based on Model Conductances. Journal of Neuroscience, 19 (14). pp. 6090-6101. ISSN 0270-6474. PMCID PMC6783079. doi:10.1523/jneurosci.19-14-06090.1999.

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Previous simulations using a realistic model of a cerebellar Purkinje cell suggested that synaptic control of somatic spiking in this cell type is mediated by voltage-gated intrinsic conductances and that inhibitory rather than excitatory synaptic inputs are more influential in controlling spike timing. In this paper, we have tested these predictions physiologically using dynamic current clamping to apply model-derived synaptic conductances to Purkinje cells in vitro. As predicted by the model, this input transformed thein vitro pattern of spiking into a different spike pattern typically observed in vivo. A net inhibitory synaptic current was required to achieve such spiking, indicating the presence of strong intrinsic depolarizing currents. Spike-triggered averaging confirmed that the length of individual intervals between spikes was correlated to the amplitude of the inhibitory conductance but was not influenced by excitatory inputs. Through repeated presentation of identical stimuli, we determined that the output spike rate was very sensitive to the relative balance of excitation and inhibition in the input conductances. In contrast, the accuracy of spike timing was dependent on input amplitude and was independent of spike rate. Thus, information could be encoded in Purkinje cell spiking in a precise spike time code and a rate code at the same time. We conclude that Purkinje cell responses to synaptic input are strongly dependent on active somatic and dendritic properties and that theories of cerebellar function likely need to incorporate single-cell dynamics to a greater degree than is customary.

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Additional Information:© 1999 Society for Neuroscience. Received Jan. 29, 1999; revised April 22, 1999; accepted April 26, 1999. This work was supported by a Sloan fellowship to D.J., by a grant from the Human Frontier Science Program to J.M.B., and by National Institute of Mental Health Grant MH57256-01 to D.J. We thank Erik De Schutter for helpful comments and simulation scripts.
Funding AgencyGrant Number
Alfred P. Sloan FoundationUNSPECIFIED
Human Frontier Science ProgramUNSPECIFIED
National Institute of Mental Health (NIMH)UNSPECIFIED
Subject Keywords:cerebellum; Purkinje cell; synapse; excitation; inhibition; dynamic clamp; modeling; in vitro; whole cell
Issue or Number:14
PubMed Central ID:PMC6783079
Record Number:CaltechAUTHORS:20191028-114852403
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Official Citation:Synaptic Control of Spiking in Cerebellar Purkinje Cells: Dynamic Current Clamp Based on Model Conductances. Dieter Jaeger, James M. Bower. Journal of Neuroscience 15 July 1999, 19 (14) 6090-6101; DOI: 10.1523/JNEUROSCI.19-14-06090.1999
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:99489
Deposited By: Tony Diaz
Deposited On:28 Oct 2019 19:49
Last Modified:16 Nov 2021 17:47

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