Pharmacological characterization of presynaptic calcium currents underlying glutamatergic transmission in the avian auditory brainstem

Sivaramakrishnan, Shobhana and Laurent, Gilles (1995) Pharmacological characterization of presynaptic calcium currents underlying glutamatergic transmission in the avian auditory brainstem. Journal of Neuroscience, 15 (10). pp. 6576-6585. ISSN 0270-6474. PMCID PMC6577991. doi:10.1523/jneurosci.15-10-06576.1995. https://resolver.caltech.edu/CaltechAUTHORS:20191101-093417244

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Abstract

We used whole-cell patch-clamp techniques on presynaptic terminals and postsynaptic neurons of the glutamatergic magnocellularis synapse in the chick auditory brainstem to study the effects of N, P, and L-type calcium channel blockers directly on presynaptic calcium currents and transmitter release. Presynaptic calcium currents and transmitter release were unaffected by nifedipine, blocked partially by omega- agatoxin IVA and completely by omega-conotoxin GVIA. The presynaptic calcium current is a low voltage-activated, noninactivating current and its block by omega-agatoxin IVA was not reversed by repeated depolarization of the presynaptic terminal. Thus, the presynaptic calcium current that underlies transmitter release at the chick magnocellularis synapse differs in some respects from N and P-type calcium currents described in vertebrate neuronal cell bodies.

Item Type:

Article

Related URLs:

URL	URL Type	Description
https://doi.org/10.1523/jneurosci.15-10-06576.1995	DOI	Article
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6577991	PubMed Central	Article

ORCID:

Author	ORCID
Laurent, Gilles	0000-0002-2296-114X

Additional Information:

© 1995 Society for Neuroscience. Received Mar. 15, 1995; revised May 25, 1995; accepted May 26, 1995. This work was supported by NIMH and Searle and McKnight Scholars awards to G.L. We thank M. Konishi, L. O. Trussell, and S. Zhang for valuable discussions during this work and M. S. Brodwick, H. Lester, E. M. Schuman, and two anonymous reviewers for critical reading of this manuscript.

Funders:

Funding Agency	Grant Number
National Institute of Mental Health (NIMH)	UNSPECIFIED
Searle Scholars Program	UNSPECIFIED
McKnight Foundation	UNSPECIFIED
NIH	UNSPECIFIED

Subject Keywords:

vertebrate CNS, chick nucleus magnocellularis, cochlear nucleus, glutamatergic transmission, presynaptic calcium current, ω-agatoxin IVA, ω-conotoxin GVIA

Issue or Number:

PubMed Central ID:

PMC6577991

DOI:

10.1523/jneurosci.15-10-06576.1995

Record Number:

CaltechAUTHORS:20191101-093417244

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:20191101-093417244

Official Citation:

Pharmacological characterization of presynaptic calcium currents underlying glutamatergic transmission in the avian auditory brainstem. S Sivaramakrishnan, G Laurent. Journal of Neuroscience 1 October 1995, 15 (10) 6576-6585; DOI: 10.1523/JNEUROSCI.15-10-06576.1995

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No commercial reproduction, distribution, display or performance rights in this work are provided.

ID Code:

99613

Collection:

CaltechAUTHORS

Deposited By:

Tony Diaz

Deposited On:

01 Nov 2019 16:40

Last Modified:

17 Feb 2022 00:26

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