CaltechAUTHORS
  A Caltech Library Service

Snapshot photoacoustic topography through an ergodic relay for high-throughput imaging of optical absorption

Li, Yang and Li, Lei and Zhu, Liren and Maslov, Konstantin and Shi, Junhui and Hu, Peng and Bo, En and Yao, Junjie and Liang, Jinyang and Wang, Lidai and Wang, Lihong V. (2020) Snapshot photoacoustic topography through an ergodic relay for high-throughput imaging of optical absorption. Nature Photonics, 14 (3). pp. 164-170. ISSN 1749-4885. PMCID PMC8223468. https://resolver.caltech.edu/CaltechAUTHORS:20191101-124901076

[img] PDF - Accepted Version
See Usage Policy.

1MB
[img] PDF (Supplementary Figs. 1–10 and Supplementary Notes 1–4) - Supplemental Material
See Usage Policy.

3MB
[img] PDF (Reporting Summary) - Supplemental Material
See Usage Policy.

70kB
[img] Video (MPEG) (Supplementary Video 1. Demonstration of PATER’s imaging mechanism) - Supplemental Material
See Usage Policy.

4MB
[img] Video (MPEG) (Supplementary Video 2. Quantification of the spatial resolution of snapshot wide-field imaging by PATER) - Supplemental Material
See Usage Policy.

366kB
[img] Video (MPEG) (Supplementary Video 3. Snapshot wide-field imaging by PATER of blood flow behind biological tissue) - Supplemental Material
See Usage Policy.

867kB
[img] Video (MPEG) (Supplementary Video 4. Snapshot wide-field functional PATER imaging of haemoglobin responses in a mouse brain to front-paw stimulations in vivo) - Supplemental Material
See Usage Policy.

1MB
[img] Video (MPEG) (Supplementary Video 5. Visualization of blood pulse wave propagation in the middle cerebral arteries) - Supplemental Material
See Usage Policy.

4MB
[img] Video (MPEG) (Supplementary Video 6. Snapshot wide-field tracking of MTCs in a tube using PATER at 660 nm light illumination) - Supplemental Material
See Usage Policy.

947kB
[img] Video (MPEG) (Supplementary Video 7. Snapshot wide-field tracking of MTCs in a mouse brain in vivo using PATER at 660 nm light illumination) - Supplemental Material
See Usage Policy.

4MB
[img] Video (MPEG) (Supplementary Video 8. Close-up slow-motion video of snapshot wide-field tracking of MTCs as shown in Supplementary Video 6) - Supplemental Material
See Usage Policy.

1MB
[img] Video (MPEG) (Supplementary Video 9. Buildup of MTC localization map. The positions of migrating MTCs in the blood vessels were tracked throughout the video from Supplementary Video 6 and superimposed) - Supplemental Material
See Usage Policy.

247kB

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20191101-124901076

Abstract

Current embodiments of photoacoustic imaging require either serial detection with a single-element ultrasonic transducer or parallel detection with an ultrasonic array, necessitating a trade-off between cost and throughput. Here, we present photoacoustic topography through an ergodic relay (PATER) for low-cost high-throughput snapshot wide-field imaging. Encoding spatial information with randomized temporal signatures through ergodicity, PATER requires only a single-element ultrasonic transducer to capture a wide-field image with a single laser shot. We applied PATER to demonstrate both functional imaging of haemodynamic responses and high-speed imaging of blood pulse wave propagation in mice in vivo. Leveraging the high frame rate of 2 kHz, PATER tracked and localized moving melanoma tumour cells in the mouse brain in vivo, which enabled flow velocity quantification and super-resolution imaging. Among the potential biomedical applications of PATER, wearable devices to monitor human vital signs in particular is envisaged.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/s41566-019-0576-2DOIArticle
https://rdcu.be/b0AwzPublisherFree ReadCube access
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223468PubMed CentralArticle
ORCID:
AuthorORCID
Li, Yang0000-0002-4939-8174
Li, Lei0000-0001-6164-2646
Zhu, Liren0000-0002-6338-9338
Maslov, Konstantin0000-0003-3408-8840
Shi, Junhui0000-0002-5741-2781
Hu, Peng0000-0002-2933-1239
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2020 Springer Nature Limited. Received: 3 April 2018; Accepted: 4 December 2019; Published: 20 January 2020. We thank J. Ballard and C. Ma for close reading of the manuscript, Y. He and C. Yeh for technical support, and P. Hai for his image superposition codes. This work was sponsored by National Institutes of Health Grants DP1 EB016986 (NIH Director’s Pioneer Award), R01 CA186567 (NIH Director’s Transformative Research Award), R01 EB016963, U01 NS090579 (NIH BRAIN Initiative) and U01 NS099717 (NIH BRAIN Initiative). Author Contributions: Y.L. and L.L. designed the study. Y.L., L.L. and K.M. built the imaging system. L.L. and Y.L. planned the experiments. Y.L., L.L., E.B. and J.Y. performed the experiments. J.S. and L.W. developed the data acquisition program. L.Z., Y.L. and L.L. developed the reconstruction algorithm. Y.L., L.L., L.Z., J.L., P.H. and J.Y. analysed the data. L.V.W. conceived the concept and supervised the project. All authors contributed to writing the manuscript. Data availability: The data that support the plots within this paper and other findings of this study are available from the corresponding author upon reasonable request and with permission from corporate collaborations. Code availability: The reconstruction algorithm and data processing methods are described in detail in the Methods. We have opted not to make the computer codes publicly available owing to corporate collaborations and pending patent applications. Competing interests: L.V.W. and K.M. have financial interests in Microphotoacoustics, Inc., CalPACT, LLC and Union Photoacoustic Technologies, Ltd, which did not support this work.
Funders:
Funding AgencyGrant Number
NIHDP1 EB016986
NIHR01 CA186567
NIHR01 EB016963
NIHU01 NS090579
NIHU01 NS099717
Issue or Number:3
PubMed Central ID:PMC8223468
Record Number:CaltechAUTHORS:20191101-124901076
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20191101-124901076
Official Citation:Li, Y., Li, L., Zhu, L. et al. Snapshot photoacoustic topography through an ergodic relay for high-throughput imaging of optical absorption. Nat. Photonics 14, 164–170 (2020). https://doi.org/10.1038/s41566-019-0576-2
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:99620
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:21 Jan 2020 19:35
Last Modified:28 Jun 2021 17:01

Repository Staff Only: item control page