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The monoclonal antibody E587 recognizes growing (new and regenerating) retinal axons in the goldfish retinotectal pathway

Vielmetter, Jost and Lottspeich, Friedrich and Stuermer, Claudia A. O. (1991) The monoclonal antibody E587 recognizes growing (new and regenerating) retinal axons in the goldfish retinotectal pathway. Journal of Neuroscience, 11 (11). pp. 3581-3593. ISSN 0270-6474. PMCID PMC6575549. doi:10.1523/jneurosci.11-11-03581.1991. https://resolver.caltech.edu/CaltechAUTHORS:20191104-083657170

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Abstract

E587 is a new monoclonal antibody against a 200 kDa cell-surface glycoprotein in the fish retinotectal pathway. The E587 antigen probably belongs to the class of cell adhesion molecules, and more specifically, to the family of L1-like molecules. The immunopurified protein is recognized by the antibody against the HNK1/L2 sugar epitope (associated with most cell adhesion molecules) and by a polyclonal antiserum against chick G4, which is related to the cell adhesion molecule L1 in mouse. Moreover the NH2-terminal sequence of E587 shows similarity with L1 and Ng-CAM. The E587 immunostaining pattern in the fish retinotectal pathway suggests that the E587 antigen is a growth- associated molecule on fish retinal axons. In fish embryos, all retinal axons are labeled. In adult fish, however, only the young axons from newly added ganglion cells carry E587 staining. After optic nerve transection (ONS) and retinal axonal regeneration, all axons reexpress the E587 antigen into their terminal processes in the tectal retinorecipient layers. The reexpression of the E587 antigen is temporally regulated, and E587 immunoreactivity declines by 7 months and disappears at 12 months after ONS. We hypothesize that the E587 antigen may mediate axon-axon associations. In its restricted appearance on young axons in normal adult fish, it may contribute to the selective fasciculation of the newest axons with young axons and thus participate in the creation of the age-related fiber organization in the fish optic nerve.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1523/jneurosci.11-11-03581.1991DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6575549PubMed CentralArticle
Additional Information:© 1991 Society for Neuroscience. Received Dec. 3, 1991; revised June 14, 1991; accepted June 27, 1991. This work was supported by the DFG Grant Stu 112/4 to C.A.O.S. We thank Marianne Wiechers for her excellent technical help and Paiz Kayyem for corrections on the manuscript. Martin Bastmeyer and Katja Wehner helped to complete the revised version of the manuscript
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Funding AgencyGrant Number
Deutsche Forschungsgemeinschaft (DFG)Stu 112/4
Issue or Number:11
PubMed Central ID:PMC6575549
DOI:10.1523/jneurosci.11-11-03581.1991
Record Number:CaltechAUTHORS:20191104-083657170
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20191104-083657170
Official Citation:The monoclonal antibody E587 recognizes growing (new and regenerating) retinal axons in the goldfish retinotectal pathway. J Vielmetter, F Lottspeich, CA Stuermer. Journal of Neuroscience 1 November 1991, 11 (11) 3581-3593; DOI: 10.1523/JNEUROSCI.11-11-03581.1991
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:99639
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Nov 2019 18:44
Last Modified:16 Nov 2021 17:48

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